BD PharmingenTM PE Mouse anti-human CD142

Description

Flow cytometric analysis of CD142 expression on activated human monocytes. Human peripheral blood mononuclear cells PBMC were stimulated 4 hr with 0.1 mug/mL lipopolysaccharide LPS; Sigma-Aldrich, Cat. No. L-2654 . The cells were harvested, washed and pre-blocked for 10 min with 5 % human AB serum. The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; dashed line histogram or PE Mouse Anti-Human CD142 antibody Cat. No. 550312/561713; solid line histogram . The fluorescence histograms showing CD142 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact activated monocytes. Flow cytometric analysis and data analysis was performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Flow cytometric analysis of CD142 expression on activated human monocytes. Human peripheral blood mononuclear cells PBMC were stimulated 4 hr with 0.1 mug/mL lipopolysaccharide LPS; Sigma-Aldrich, Cat. No. L-2654 . The cells were harvested, washed and pre-blocked for 10 min with 5 % human AB serum. The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; dashed line histogram or PE Mouse Anti-Human CD142 antibody Cat. No. 550312/561713; solid line histogram . The fluorescence histograms showing CD142 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact activated monocytes. Flow cytometric analysis and data analysis was performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Show More Flow cytometric analysis of CD142 expression on activated human monocytes. Human peripheral blood mononuclear cells PBMC were stimulated 4 hr with 0.1 mug/mL lipopolysaccharide LPS; Sigma-Aldrich, Cat. No. L-2654 . The cells were harvested, washed and pre-blocked for 10 min with 5 % human AB serum. The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; dashed line histogram or PE Mouse Anti-Human CD142 antibody Cat. No. 550312/561713; solid line histogram . The fluorescence histograms showing CD142 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact activated monocytes. Flow cytometric analysis and data analysis was performed using a BD FACSCelesta Flow Cytometer System and FlowJo software.