BD PharmingenTM PE Mouse Anti-Human CD1e

Description

Flow cytometric analysis of CD1e expression in human Jurkat cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Perm/Wash Buffer Cat. No. 554723 . Cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat No. 554680, dashed line histogram or PE Mouse Anti-Human CD1e antibody Cat No. 567271/567272; solid line histogram at 0.125 mug/test. The fluorescence histogram showing intracellular CD1e expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD1e expression in human Jurkat cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Perm/Wash Buffer Cat. No. 554723 . Cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat No. 554680, dashed line histogram or PE Mouse Anti-Human CD1e antibody Cat No. 567271/567272; solid line histogram at 0.125 mug/test. The fluorescence histogram showing intracellular CD1e expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD1e expression in human Jurkat cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Perm/Wash Buffer Cat. No. 554723 . Cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat No. 554680, dashed line histogram or PE Mouse Anti-Human CD1e antibody Cat No. 567271/567272; solid line histogram at 0.125 mug/test. The fluorescence histogram showing intracellular CD1e expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.