BD PharmingenTM PE Mouse Anti-Human CD200

Description

Multiparameter flow cytometric analysis of CD200 expression on human peripheral blood leucocyte populations. Whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Plots or PE Mouse Anti-Human CD200 antibody Cat. No. 552475/561762; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of CD200 or Ig Isotype control staining versus side-light scatter SSC-A signals were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression of CD200 or Ig Isotype control staining versus CD3 signals were derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Multiparameter flow cytometric analysis of CD200 expression on human peripheral blood leucocyte populations. Whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Plots or PE Mouse Anti-Human CD200 antibody Cat. No. 552475/561762; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of CD200 or Ig Isotype control staining versus side-light scatter SSC-A signals were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression of CD200 or Ig Isotype control staining versus CD3 signals were derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Show More Multiparameter flow cytometric analysis of CD200 expression on human peripheral blood leucocyte populations. Whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Plots or PE Mouse Anti-Human CD200 antibody Cat. No. 552475/561762; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of CD200 or Ig Isotype control staining versus side-light scatter SSC-A signals were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression of CD200 or Ig Isotype control staining versus CD3 signals were derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes.