
562972 ·
BDB Bioscience · Cat: 562972
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multicolor flow cytometric analysis of CD203c expression on human peripheral blood cells. Human whole blood was stained with Mouse PerCP-Cy5.5 Anti-Human CD123 antibody Cat. No. 560904/558714 and either PE Mouse IgG1 kappa Isotype Control Cat. No. 554680; Left Panel or PE Mouse Anti-Human CD203c antibody Cat. No. 562972; Right Panel . Whole blood samples were then treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes and fix the remaining leucocytes. The two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus CD203c or Ig Isotype control staining for gated events with the forward and side-light scattering characteristics of intact lymphoid cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. A target acquisition of at least 500 events with low side-light scatter characteristics and bright CD123 expression were included. Multicolor flow cytometric analysis of CD203c expression on human peripheral blood cells. Human whole blood was stained with Mouse PerCP-Cy5.5 Anti-Human CD123 antibody Cat. No. 560904/558714 and either PE Mouse IgG1 kappa Isotype Control Cat. No. 554680; Left Panel or PE Mouse Anti-Human CD203c antibody Cat. No. 562972; Right Panel . Whole blood samples were then treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes and fix the remaining leucocytes. The two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus CD203c or Ig Isotype control staining for gated events with the forward and side-light scattering characteristics of intact lymphoid cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. A target acquisition of at least 500 events with low side-light scatter characteristics and bright CD123 expression were included. Multicolor flow cytometric analysis of CD203c expression on human peripheral blood cells. Human whole blood was stained with Mouse PerCP-Cy5.5 Anti-Human CD123 antibody Cat. No. 560904/558714 and either PE Mouse IgG1 kappa Isotype Control Cat. No. 554680; Left Panel or PE Mouse Anti-Human CD203c antibody Cat. No. 562972; Right Panel . Whole blood samples were then treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes and fix the remaining leucocytes. The two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus CD203c or Ig Isotype control staining for gated events with the forward and side-light scattering characteristics of intact lymphoid cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. A target acquisition of at least 500 events with low side-light scatter characteristics and bright CD123 expression were included. Show More Multicolor flow cytometric analysis of CD203c expression on human peripheral blood cells. Human whole blood was stained with Mouse PerCP-Cy5.5 Anti-Human CD123 antibody Cat. No. 560904/558714 and either PE Mouse IgG1 kappa Isotype Control Cat. No. 554680; Left Panel or PE Mouse Anti-Human CD203c antibody Cat. No. 562972; Right Panel . Whole blood samples were then treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes and fix the remaining leucocytes. The two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus CD203c or Ig Isotype control staining for gated events with the forward and side-light scattering characteristics of intact lymphoid cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. A target acquisition of at least 500 events with low side-light scatter characteristics and bright CD123 expression were included. Show More Multicolor flow cytometric analysis of CD203c expression on human peripheral blood cells. Human whole blood was stained with Mouse PerCP-Cy5.5 Anti-Human CD123 antibody Cat. No. 560904/558714 and either PE Mouse IgG1 kappa Isotype Control Cat. No. 554680; Left Panel or PE Mouse Anti-Human CD203c antibody Cat. No. 562972; Right Panel . Whole blood samples were then treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes and fix the remaining leucocytes. The two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus CD203c or Ig Isotype control staining for gated events with the forward and side-light scattering characteristics of intact lymphoid cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. A target acquisition of at least 500 events with low side-light scatter characteristics and bright CD123 expression were included. Multicolor flow cytometric analysis of CD203c expression on human peripheral blood cells. Human whole blood was stained with Mouse PerCP-Cy5.5 Anti-Human CD123 antibody Cat. No. 560904/558714 and either PE Mouse IgG1 kappa Isotype Control Cat. No. 554680; Left Panel or PE Mouse Anti-Human CD203c antibody Cat. No. 562972; Right Panel . Whole blood samples were then treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes and fix the remaining leucocytes. The two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus CD203c or Ig Isotype control staining for gated events with the forward and side-light scattering characteristics of intact lymphoid cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. A target acquisition of at least 500 events with low side-light scatter characteristics and bright CD123 expression were included.