BD PharmingenTM PE Mouse Anti-Human CD301 (CLEC10A)

Description

Flow cytometric analysis of CD301 CLEC10A expression on Human peripheral blood CD1c+ dendritic cells. Human peripheral blood mononuclear cells PBMC were stained in CaCl2-supplemented HBSS, with Alexa Fluor 647 Mouse Anti-Human CD11c Cat. No. 565912 and BD OptiBuild BV650 Mouse Anti-Human CD1c Cat. No. 742749 antibodies and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human CD301 CLEC10A antibody Cat. No. 572154/572155; solid line histogram . The fluorescence histogram showing the expression of CD301 CLEC10A or Ig Isotype control staining was derived from CD1c+CD11c+ gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD301 CLEC10A expression on Human peripheral blood leukocyte populations. Human peripheral blood cells were stained with Alexa Fluor 647 Mouse Anti-Human CD11c antibody Cat. No. 565912 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human CD301 CLEC10A antibody Cat. No. 572154/572155 as indicated. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Panel A Human peripheral blood leukocyte populations . The bivariate pseudocolor density plot showing the correlated expression of CD301 CLEC10A or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Panel B - Human peripheral blood monocytes . The bivariate pseudocolor density plot showing the correlated expression of CD301 CLEC10A or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD301 CLEC10A expression on Human peripheral blood CD1c+ dendritic cells. Human peripheral blood mononuclear cells PBMC were stained in CaCl2-supplemented HBSS, with Alexa Fluor 647 Mouse Anti-Human CD11c Cat. No. 565912 and BD OptiBuild BV650 Mouse Anti-Human CD1c Cat. No. 742749 antibodies and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human CD301 CLEC10A antibody Cat. No. 572154/572155; solid line histogram . The fluorescence histogram showing the expression of CD301 CLEC10A or Ig Isotype control staining was derived from CD1c+CD11c+ gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD301 CLEC10A expression on Human peripheral blood leukocyte populations. Human peripheral blood cells were stained with Alexa Fluor 647 Mouse Anti-Human CD11c antibody Cat. No. 565912 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human CD301 CLEC10A antibody Cat. No. 572154/572155 as indicated. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Panel A Human peripheral blood leukocyte populations . The bivariate pseudocolor density plot showing the correlated expression of CD301 CLEC10A or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Panel B - Human peripheral blood monocytes . The bivariate pseudocolor density plot showing the correlated expression of CD301 CLEC10A or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD301 CLEC10A expression on Human peripheral blood CD1c+ dendritic cells. Human peripheral blood mononuclear cells PBMC were stained in CaCl2-supplemented HBSS, with Alexa Fluor 647 Mouse Anti-Human CD11c Cat. No. 565912 and BD OptiBuild BV650 Mouse Anti-Human CD1c Cat. No. 742749 antibodies and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human CD301 CLEC10A antibody Cat. No. 572154/572155; solid line histogram . The fluorescence histogram showing the expression of CD301 CLEC10A or Ig Isotype control staining was derived from CD1c+CD11c+ gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD301 CLEC10A expression on Human peripheral blood leukocyte populations. Human peripheral blood cells were stained with Alexa Fluor 647 Mouse Anti-Human CD11c antibody Cat. No. 565912 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human CD301 CLEC10A antibody Cat. No. 572154/572155 as indicated. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Panel A Human peripheral blood leukocyte populations . The bivariate pseudocolor density plot showing the correlated expression of CD301 CLEC10A or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Panel B - Human peripheral blood monocytes . The bivariate pseudocolor density plot showing the correlated expression of CD301 CLEC10A or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.