BD PharmingenTM PE Mouse Anti-Human CD319 (CRACC)

Description

Multiparameter flow cytometric analysis of CD319 CRACC expression on human peripheral blood leucocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 564000/555899 and either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plots or PE Mouse Anti-Human CD319 CRACC antibody Cat. No. 566840; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Bivariate pseudocolor density plots showing the correlated expression of CD319 CRACC or Ig Isotype control staining versus side light-scatter signals were derived from gated events with the forward and side light scatter characteristics of viable leucocyte populations Top Plots . The bivariate plots showing the correlated expression of CD319 CRACC or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light scatter characteristics of viable lymphocytes Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD319 CRACC expression on human peripheral blood leucocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 564000/555899 and either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plots or PE Mouse Anti-Human CD319 CRACC antibody Cat. No. 566840; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Bivariate pseudocolor density plots showing the correlated expression of CD319 CRACC or Ig Isotype control staining versus side light-scatter signals were derived from gated events with the forward and side light scatter characteristics of viable leucocyte populations Top Plots . The bivariate plots showing the correlated expression of CD319 CRACC or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light scatter characteristics of viable lymphocytes Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD319 CRACC expression on human peripheral blood leucocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 564000/555899 and either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plots or PE Mouse Anti-Human CD319 CRACC antibody Cat. No. 566840; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Bivariate pseudocolor density plots showing the correlated expression of CD319 CRACC or Ig Isotype control staining versus side light-scatter signals were derived from gated events with the forward and side light scatter characteristics of viable leucocyte populations Top Plots . The bivariate plots showing the correlated expression of CD319 CRACC or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light scatter characteristics of viable lymphocytes Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.