BD PharmingenTM PE Mouse Anti-Human CD324 (E-Cadherin)

Description

Flow Cytometric Analysis of CD324 E-cadherin on human breast adenocarcinoma left and human embryonic stem ES cells right . Human breast adenocarcinoma cell line MCF-7 ATCC, HTB-22 and H9 human ES Cells WiCell, Madison, WI were harvested with Cell Dissociation Buffer Life Technologies . Please note that the epitope is sensitive to Accutase detachment. Cells were stained with either PE Mouse IgG1, k isotype control Cat. No. 554680; dashed line or PE Mouse anti-human CD324 antibody Cat. No. 562870; solid line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of MCF-7 or human ES cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Staining with this antibody can also performed on cells that have been fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . However, the brightness of the staining decreases after cell fixation and permeabilization. Flow Cytometric Analysis of CD324 E-cadherin on human breast adenocarcinoma left and human embryonic stem ES cells right . Human breast adenocarcinoma cell line MCF-7 ATCC, HTB-22 and H9 human ES Cells WiCell, Madison, WI were harvested with Cell Dissociation Buffer Life Technologies . Please note that the epitope is sensitive to Accutase detachment. Cells were stained with either PE Mouse IgG1, k isotype control Cat. No. 554680; dashed line or PE Mouse anti-human CD324 antibody Cat. No. 562870; solid line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of MCF-7 or human ES cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Staining with this antibody can also performed on cells that have been fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . However, the brightness of the staining decreases after cell fixation and permeabilization. Flow Cytometric Analysis of CD324 E-cadherin on human breast adenocarcinoma left and human embryonic stem ES cells right . Human breast adenocarcinoma cell line MCF-7 ATCC, HTB-22 and H9 human ES Cells WiCell, Madison, WI were harvested with Cell Dissociation Buffer Life Technologies . Please note that the epitope is sensitive to Accutase detachment. Cells were stained with either PE Mouse IgG1, k isotype control Cat. No. 554680; dashed line or PE Mouse anti-human CD324 antibody Cat. No. 562870; solid line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of MCF-7 or human ES cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Staining with this antibody can also performed on cells that have been fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . However, the brightness of the staining decreases after cell fixation and permeabilization. Show More Flow Cytometric Analysis of CD324 E-cadherin on human breast adenocarcinoma left and human embryonic stem ES cells right . Human breast adenocarcinoma cell line MCF-7 ATCC, HTB-22 and H9 human ES Cells WiCell, Madison, WI were harvested with Cell Dissociation Buffer Life Technologies . Please note that the epitope is sensitive to Accutase detachment. Cells were stained with either PE Mouse IgG1, k isotype control Cat. No. 554680; dashed line or PE Mouse anti-human CD324 antibody Cat. No. 562870; solid line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of MCF-7 or human ES cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Staining with this antibody can also performed on cells that have been fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . However, the brightness of the staining decreases after cell fixation and permeabilization. Show More Flow Cytometric Analysis of CD324 E-cadherin on human breast adenocarcinoma left and human embryonic stem ES cells right . Human breast adenocarcinoma cell line MCF-7 ATCC, HTB-22 and H9 human ES Cells WiCell, Madison, WI were harvested with Cell Dissociation Buffer Life Technologies . Please note that the epitope is sensitive to Accutase detachment. Cells were stained with either PE Mouse IgG1, k isotype control Cat. No. 554680; dashed line or PE Mouse anti-human CD324 antibody Cat. No. 562870; solid line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of MCF-7 or human ES cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Staining with this antibody can also performed on cells that have been fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . However, the brightness of the staining decreases after cell fixation and permeabilization. Flow Cytometric Analysis of CD324 E-cadherin on human breast adenocarcinoma left and human embryonic stem ES cells right . Human breast adenocarcinoma cell line MCF-7 ATCC, HTB-22 and H9 human ES Cells WiCell, Madison, WI were harvested with Cell Dissociation Buffer Life Technologies . Please note that the epitope is sensitive to Accutase detachment. Cells were stained with either PE Mouse IgG1, k isotype control Cat. No. 554680; dashed line or PE Mouse anti-human CD324 antibody Cat. No. 562870; solid line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of MCF-7 or human ES cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Staining with this antibody can also performed on cells that have been fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . However, the brightness of the staining decreases after cell fixation and permeabilization.