BD PharmingenTM PE Mouse Anti-Human CD36

Description

Flow cytometric analysis of CD36 expression on Human peripheral blood platelets. Platelets were isolated from fresh whole blood and fixed with 2 % formaldehyde. After washing, the fixed platelets were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human CD36 antibody Cat. No. 570214/570215; solid line histogram . The fluorescence histogram showing CD36 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact platelets. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD36 expression on Human peripheral blood leukocyte populations. Platelet-depleted whole blood was stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human CD36 antibody Cat. No. 570214/570215; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The pseudocolor density plot showing the correlated expression of CD36 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD36 expression on Human peripheral blood platelets. Platelets were isolated from fresh whole blood and fixed with 2 % formaldehyde. After washing, the fixed platelets were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human CD36 antibody Cat. No. 570214/570215; solid line histogram . The fluorescence histogram showing CD36 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact platelets. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD36 expression on Human peripheral blood leukocyte populations. Platelet-depleted whole blood was stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human CD36 antibody Cat. No. 570214/570215; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The pseudocolor density plot showing the correlated expression of CD36 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD36 expression on Human peripheral blood platelets. Platelets were isolated from fresh whole blood and fixed with 2 % formaldehyde. After washing, the fixed platelets were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human CD36 antibody Cat. No. 570214/570215; solid line histogram . The fluorescence histogram showing CD36 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact platelets. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD36 expression on Human peripheral blood leukocyte populations. Platelet-depleted whole blood was stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human CD36 antibody Cat. No. 570214/570215; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The pseudocolor density plot showing the correlated expression of CD36 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.