BD PharmingenTM PE Mouse Anti-Human CD39

Description

Flow cytometric analysis of CD39 expression on human peripheral blood leucocyte populations. Upper Plots: Whole blood was stained with either PE Mouse IgG1, kappa Isotype Control Cat No. 554680, Left Plot or PE Mouse Anti-Human CD39 antibody Cat No. 567156/567157, Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat No. 555899 . A bivariate pseudocolor density plot showing the correlated expression of CD39 or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Lower Plots: Human peripheral blood mononuclear cells PBMC were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , PE-Cy7 Mouse Anti-Human CD25 Cat. No. 557741/560920/561405 , Alexa Fluor 647 Mouse Anti-Human CD127 Cat No. 558598/560905 antibodies, and either PE Mouse IgG1, kappa Isotype Control dashed line histogram or PE Mouse Anti-Human CD39 solid line histogram . Bivariate pseudocolor density plots showing the coexpressed levels of CD25 versus CD127 by viable light scatter-gated CD4+ T cells Left Plot were further gated to reveal CD39 expression or Ig Isotype control staining Right Plot on CD4+CD25+CD127low T cells ie, cells with a Regulatory T cell immunophenotype as shown. Flow cytometric analysis was performed using a BD FACSCelelsta or LSRFortessa X-20 Flow Cytometer System and FlowJo software. Flow cytometric analysis of CD39 expression on human peripheral blood leucocyte populations. Upper Plots: Whole blood was stained with either PE Mouse IgG1, kappa Isotype Control Cat No. 554680, Left Plot or PE Mouse Anti-Human CD39 antibody Cat No. 567156/567157, Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat No. 555899 . A bivariate pseudocolor density plot showing the correlated expression of CD39 or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Lower Plots: Human peripheral blood mononuclear cells PBMC were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , PE-Cy7 Mouse Anti-Human CD25 Cat. No. 557741/560920/561405 , Alexa Fluor 647 Mouse Anti-Human CD127 Cat No. 558598/560905 antibodies, and either PE Mouse IgG1, kappa Isotype Control dashed line histogram or PE Mouse Anti-Human CD39 solid line histogram . Bivariate pseudocolor density plots showing the coexpressed levels of CD25 versus CD127 by viable light scatter-gated CD4+ T cells Left Plot were further gated to reveal CD39 expression or Ig Isotype control staining Right Plot on CD4+CD25+CD127low T cells ie, cells with a Regulatory T cell immunophenotype as shown. Flow cytometric analysis was performed using a BD FACSCelelsta or LSRFortessa X-20 Flow Cytometer System and FlowJo software. Show More Flow cytometric analysis of CD39 expression on human peripheral blood leucocyte populations. Upper Plots: Whole blood was stained with either PE Mouse IgG1, kappa Isotype Control Cat No. 554680, Left Plot or PE Mouse Anti-Human CD39 antibody Cat No. 567156/567157, Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat No. 555899 . A bivariate pseudocolor density plot showing the correlated expression of CD39 or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Lower Plots: Human peripheral blood mononuclear cells PBMC were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , PE-Cy7 Mouse Anti-Human CD25 Cat. No. 557741/560920/561405 , Alexa Fluor 647 Mouse Anti-Human CD127 Cat No. 558598/560905 antibodies, and either PE Mouse IgG1, kappa Isotype Control dashed line histogram or PE Mouse Anti-Human CD39 solid line histogram . Bivariate pseudocolor density plots showing the coexpressed levels of CD25 versus CD127 by viable light scatter-gated CD4+ T cells Left Plot were further gated to reveal CD39 expression or Ig Isotype control staining Right Plot on CD4+CD25+CD127low T cells ie, cells with a Regulatory T cell immunophenotype as shown. Flow cytometric analysis was performed using a BD FACSCelelsta or LSRFortessa X-20 Flow Cytometer System and FlowJo software.