BD PharmingenTM PE Mouse Anti-Human CD59

Description

Flow cytometric analysis of CD59 expression on peripheral blood leucocytes. Human or rhesus whole blood was lysed with BD FACS Lysing Solution Cat. No. 349202 , then preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 555574; dashed line histogram or PE Mouse Anti-Human CD59 Cat. No. 555764/557141/560953; solid line histogram . Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of human lymphocytes Left Panel or rhesus granulocytes Right Panel . Flow cytometry was performed on a BD LSRFortessa X-20 system. Flow cytometric analysis of CD59 expression on peripheral blood leucocytes. Human or rhesus whole blood was lysed with BD FACS Lysing Solution Cat. No. 349202 , then preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 555574; dashed line histogram or PE Mouse Anti-Human CD59 Cat. No. 555764/557141/560953; solid line histogram . Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of human lymphocytes Left Panel or rhesus granulocytes Right Panel . Flow cytometry was performed on a BD LSRFortessa X-20 system. Flow cytometric analysis of CD59 expression on peripheral blood leucocytes. Human or rhesus whole blood was lysed with BD FACS Lysing Solution Cat. No. 349202 , then preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 555574; dashed line histogram or PE Mouse Anti-Human CD59 Cat. No. 555764/557141/560953; solid line histogram . Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of human lymphocytes Left Panel or rhesus granulocytes Right Panel . Flow cytometry was performed on a BD LSRFortessa X-20 system. Show More Flow cytometric analysis of CD59 expression on peripheral blood leucocytes. Human or rhesus whole blood was lysed with BD FACS Lysing Solution Cat. No. 349202 , then preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 555574; dashed line histogram or PE Mouse Anti-Human CD59 Cat. No. 555764/557141/560953; solid line histogram . Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of human lymphocytes Left Panel or rhesus granulocytes Right Panel . Flow cytometry was performed on a BD LSRFortessa X-20 system. Show More Flow cytometric analysis of CD59 expression on peripheral blood leucocytes. Human or rhesus whole blood was lysed with BD FACS Lysing Solution Cat. No. 349202 , then preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 555574; dashed line histogram or PE Mouse Anti-Human CD59 Cat. No. 555764/557141/560953; solid line histogram . Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of human lymphocytes Left Panel or rhesus granulocytes Right Panel . Flow cytometry was performed on a BD LSRFortessa X-20 system. Flow cytometric analysis of CD59 expression on peripheral blood leucocytes. Human or rhesus whole blood was lysed with BD FACS Lysing Solution Cat. No. 349202 , then preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 555574; dashed line histogram or PE Mouse Anti-Human CD59 Cat. No. 555764/557141/560953; solid line histogram . Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of human lymphocytes Left Panel or rhesus granulocytes Right Panel . Flow cytometry was performed on a BD LSRFortessa X-20 system.