BD PharmingenTM PE Mouse Anti-Human CD83

Description

Flow cytometric analysis of CD83 expression on cultured human dendritic cells. Human peripheral blood monocytes were treated with 20 ng/ml of Recombinant Human IL-4 Cat. No. 554605 , and 20 ng/ml Recombinant Human GM-CSF Cat. No. 550068 proteins for 7 days at 37C. In addition, 20 ng/ml Recombinant Human TNF Cat. No. 554618 was added for the last two days of culture. The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; dashed line histogram or PE Mouse Anti-Human CD83 antibody Cat. No. 556855/561959; solid line histogram . The fluorescence histogram showing CD83 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytomtery was performed on a BD FACScan system. Flow cytometric analysis of CD83 expression on cultured human dendritic cells. Human peripheral blood monocytes were treated with 20 ng/ml of Recombinant Human IL-4 Cat. No. 554605 , and 20 ng/ml Recombinant Human GM-CSF Cat. No. 550068 proteins for 7 days at 37C. In addition, 20 ng/ml Recombinant Human TNF Cat. No. 554618 was added for the last two days of culture. The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; dashed line histogram or PE Mouse Anti-Human CD83 antibody Cat. No. 556855/561959; solid line histogram . The fluorescence histogram showing CD83 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytomtery was performed on a BD FACScan system. Show More Flow cytometric analysis of CD83 expression on cultured human dendritic cells. Human peripheral blood monocytes were treated with 20 ng/ml of Recombinant Human IL-4 Cat. No. 554605 , and 20 ng/ml Recombinant Human GM-CSF Cat. No. 550068 proteins for 7 days at 37C. In addition, 20 ng/ml Recombinant Human TNF Cat. No. 554618 was added for the last two days of culture. The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; dashed line histogram or PE Mouse Anti-Human CD83 antibody Cat. No. 556855/561959; solid line histogram . The fluorescence histogram showing CD83 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytomtery was performed on a BD FACScan system.