BD PharmingenTM PE Mouse Anti-Human CD85k

Description

Multiparameter flow cytometric analysis of CD85k expression on human peripheral blood leucocytes. Human whole blood was stained with PerCP-Cy5.5 Mouse Anti-Human CD123 Cat. No. 558714 and APC Mouse Anti-Human ILT7 CD85g Cat. No. 562355 antibodies and either PE Mouse IgG1 Kappa Isotype Control Cat. No. 551436 or PE Mouse Anti-Human CD85k antibody Cat. No. 564181 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression of CD123 versus Ig Isotype control staining Plot A or CD85k Plot B and Plot C showing CD85k versus ILT7 CD85g coexpression patterns were derived from gated events with the forward and side scattered SSC-A light characteristics of intact peripheral blood mononuclear cells. The two-parameter flow cytometric plot showing CD85k expression versus side light-scatter signals Plot D was derived from gated events with the light scattering characteristics of intact peripheral blood leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and BD FACSDiva Software 8.0. Multiparameter flow cytometric analysis of CD85k expression on human peripheral blood leucocytes. Human whole blood was stained with PerCP-Cy5.5 Mouse Anti-Human CD123 Cat. No. 558714 and APC Mouse Anti-Human ILT7 CD85g Cat. No. 562355 antibodies and either PE Mouse IgG1 Kappa Isotype Control Cat. No. 551436 or PE Mouse Anti-Human CD85k antibody Cat. No. 564181 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression of CD123 versus Ig Isotype control staining Plot A or CD85k Plot B and Plot C showing CD85k versus ILT7 CD85g coexpression patterns were derived from gated events with the forward and side scattered SSC-A light characteristics of intact peripheral blood mononuclear cells. The two-parameter flow cytometric plot showing CD85k expression versus side light-scatter signals Plot D was derived from gated events with the light scattering characteristics of intact peripheral blood leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and BD FACSDiva Software 8.0. Show More Multiparameter flow cytometric analysis of CD85k expression on human peripheral blood leucocytes. Human whole blood was stained with PerCP-Cy5.5 Mouse Anti-Human CD123 Cat. No. 558714 and APC Mouse Anti-Human ILT7 CD85g Cat. No. 562355 antibodies and either PE Mouse IgG1 Kappa Isotype Control Cat. No. 551436 or PE Mouse Anti-Human CD85k antibody Cat. No. 564181 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression of CD123 versus Ig Isotype control staining Plot A or CD85k Plot B and Plot C showing CD85k versus ILT7 CD85g coexpression patterns were derived from gated events with the forward and side scattered SSC-A light characteristics of intact peripheral blood mononuclear cells. The two-parameter flow cytometric plot showing CD85k expression versus side light-scatter signals Plot D was derived from gated events with the light scattering characteristics of intact peripheral blood leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and BD FACSDiva Software 8.0.