BD PharmingenTM PE Mouse Anti-Human CDX-2

Description

Flow cytometric analysis of CDX-2 expression on human DLD-1 cells. Cells from the human DLD-1 Colorectal adenocarcinoma, ATCC CCL-221 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . The cells were then permeabilized and stained in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-CDX-2 antibody Cat. No. 563428; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side scattered-light characteristics of intact cells. This antibody also works with cells permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CDX-2 expression on human DLD-1 cells. Cells from the human DLD-1 Colorectal adenocarcinoma, ATCC CCL-221 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . The cells were then permeabilized and stained in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-CDX-2 antibody Cat. No. 563428; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side scattered-light characteristics of intact cells. This antibody also works with cells permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CDX-2 expression on human DLD-1 cells. Cells from the human DLD-1 Colorectal adenocarcinoma, ATCC CCL-221 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . The cells were then permeabilized and stained in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-CDX-2 antibody Cat. No. 563428; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side scattered-light characteristics of intact cells. This antibody also works with cells permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.