BD PharmingenTM PE Mouse Anti-Human COX-2

Description

Multiparameter flow cytometric analysis of COX-2 expression in activated human CD14+ monocytes. Human peripheral blood mononuclear cells were either cultured alone Untreated; Left Panel or cultured LPS-Treated; Right Panel for 5 hours in the presence of lipopolysaccharide LPS; 1 mug/ml; Sigma, Cat. No. L2654 . Cells in suspension and adherent cells collected using BD Accutase Cell Detachment Solution; Cat. No. 561527 were harvested, washed, and stained with PerCP-Cy5.5 Mouse Anti-Human CD14 antibody Cat. No. 562692 . The cells were then washed, fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human COX-2 antibody Cat. No. 565125 , as indicated. Two-parameter flow cytometric dot plots showing the correlated expression of forward scattered-light signals versus COX-2 or Ig Isotype control staining were derived from CD14-positive gated events with the forward and side scattered-light characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multiparameter flow cytometric analysis of COX-2 expression in activated human CD14+ monocytes. Human peripheral blood mononuclear cells were either cultured alone Untreated; Left Panel or cultured LPS-Treated; Right Panel for 5 hours in the presence of lipopolysaccharide LPS; 1 mug/ml; Sigma, Cat. No. L2654 . Cells in suspension and adherent cells collected using BD Accutase Cell Detachment Solution; Cat. No. 561527 were harvested, washed, and stained with PerCP-Cy5.5 Mouse Anti-Human CD14 antibody Cat. No. 562692 . The cells were then washed, fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human COX-2 antibody Cat. No. 565125 , as indicated. Two-parameter flow cytometric dot plots showing the correlated expression of forward scattered-light signals versus COX-2 or Ig Isotype control staining were derived from CD14-positive gated events with the forward and side scattered-light characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multiparameter flow cytometric analysis of COX-2 expression in activated human CD14+ monocytes. Human peripheral blood mononuclear cells were either cultured alone Untreated; Left Panel or cultured LPS-Treated; Right Panel for 5 hours in the presence of lipopolysaccharide LPS; 1 mug/ml; Sigma, Cat. No. L2654 . Cells in suspension and adherent cells collected using BD Accutase Cell Detachment Solution; Cat. No. 561527 were harvested, washed, and stained with PerCP-Cy5.5 Mouse Anti-Human CD14 antibody Cat. No. 562692 . The cells were then washed, fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human COX-2 antibody Cat. No. 565125 , as indicated. Two-parameter flow cytometric dot plots showing the correlated expression of forward scattered-light signals versus COX-2 or Ig Isotype control staining were derived from CD14-positive gated events with the forward and side scattered-light characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.