BD PharmingenTM PE Mouse Anti-Human DNAM-1 (CD226)

Description

Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with Alexa Fluor 647 Mouse Anti-Human CD3 Cat. No. 557706 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567358; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Bivariate pseudocolor density plots showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 were derived from gated events based on the light scattering characteristics of viable lymphocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with Alexa Fluor 647 Mouse Anti-Human CD3 Cat. No. 557706 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567358; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Bivariate pseudocolor density plots showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 were derived from gated events based on the light scattering characteristics of viable lymphocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with Alexa Fluor 647 Mouse Anti-Human CD3 Cat. No. 557706 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567358; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Bivariate pseudocolor density plots showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 were derived from gated events based on the light scattering characteristics of viable lymphocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with Alexa Fluor 647 Mouse Anti-Human CD3 Cat. No. 557706 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567358; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Bivariate pseudocolor density plots showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 were derived from gated events based on the light scattering characteristics of viable lymphocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with Alexa Fluor 647 Mouse Anti-Human CD3 Cat. No. 557706 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567358; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Bivariate pseudocolor density plots showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 were derived from gated events based on the light scattering characteristics of viable lymphocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with Alexa Fluor 647 Mouse Anti-Human CD3 Cat. No. 557706 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567358; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Bivariate pseudocolor density plots showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 were derived from gated events based on the light scattering characteristics of viable lymphocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.