BD PharmingenTM PE Mouse Anti-Human FCRL5 (CD307e)

Description

Flow cytometric analysis of FCRL5 CD307e expression on transfectants and tonsillar B cells. Left Plot: Non-transfected Dotted line histogram and Human FCRL5-transfected Solid line histogram P815 cells were surface stained with PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734 at 0.5 mug/test. Flow cytometric fluorescence histograms showing the expression of FCRL5 CD307e on non-transfected or Human FCRL5-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Middle and Right Plots: Tonsil cells were treated with an Fc receptor blocker and stained with APC Mouse Anti-Human CD19 Cat. No. 561742 or 555415 and either PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734; Middle Plot or PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of FCRL5 CD307e or Ig isotype control staining versus Human CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCalibur Flow Cytometer System and FlowJo software. Data courtesy of Dr. Marco Colonna, Washington University School of Medicine. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of FCRL5 CD307e expression on transfectants and tonsillar B cells. Left Plot: Non-transfected Dotted line histogram and Human FCRL5-transfected Solid line histogram P815 cells were surface stained with PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734 at 0.5 mug/test. Flow cytometric fluorescence histograms showing the expression of FCRL5 CD307e on non-transfected or Human FCRL5-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Middle and Right Plots: Tonsil cells were treated with an Fc receptor blocker and stained with APC Mouse Anti-Human CD19 Cat. No. 561742 or 555415 and either PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734; Middle Plot or PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of FCRL5 CD307e or Ig isotype control staining versus Human CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCalibur Flow Cytometer System and FlowJo software. Data courtesy of Dr. Marco Colonna, Washington University School of Medicine. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of FCRL5 CD307e expression on transfectants and tonsillar B cells. Left Plot: Non-transfected Dotted line histogram and Human FCRL5-transfected Solid line histogram P815 cells were surface stained with PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734 at 0.5 mug/test. Flow cytometric fluorescence histograms showing the expression of FCRL5 CD307e on non-transfected or Human FCRL5-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Middle and Right Plots: Tonsil cells were treated with an Fc receptor blocker and stained with APC Mouse Anti-Human CD19 Cat. No. 561742 or 555415 and either PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734; Middle Plot or PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of FCRL5 CD307e or Ig isotype control staining versus Human CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCalibur Flow Cytometer System and FlowJo software. Data courtesy of Dr. Marco Colonna, Washington University School of Medicine. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of FCRL5 CD307e expression on transfectants and tonsillar B cells. Left Plot: Non-transfected Dotted line histogram and Human FCRL5-transfected Solid line histogram P815 cells were surface stained with PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734 at 0.5 mug/test. Flow cytometric fluorescence histograms showing the expression of FCRL5 CD307e on non-transfected or Human FCRL5-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Middle and Right Plots: Tonsil cells were treated with an Fc receptor blocker and stained with APC Mouse Anti-Human CD19 Cat. No. 561742 or 555415 and either PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734; Middle Plot or PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of FCRL5 CD307e or Ig isotype control staining versus Human CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCalibur Flow Cytometer System and FlowJo software. Data courtesy of Dr. Marco Colonna, Washington University School of Medicine. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of FCRL5 CD307e expression on transfectants and tonsillar B cells. Left Plot: Non-transfected Dotted line histogram and Human FCRL5-transfected Solid line histogram P815 cells were surface stained with PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734 at 0.5 mug/test. Flow cytometric fluorescence histograms showing the expression of FCRL5 CD307e on non-transfected or Human FCRL5-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Middle and Right Plots: Tonsil cells were treated with an Fc receptor blocker and stained with APC Mouse Anti-Human CD19 Cat. No. 561742 or 555415 and either PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734; Middle Plot or PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of FCRL5 CD307e or Ig isotype control staining versus Human CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCalibur Flow Cytometer System and FlowJo software. Data courtesy of Dr. Marco Colonna, Washington University School of Medicine. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of FCRL5 CD307e expression on transfectants and tonsillar B cells. Left Plot: Non-transfected Dotted line histogram and Human FCRL5-transfected Solid line histogram P815 cells were surface stained with PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734 at 0.5 mug/test. Flow cytometric fluorescence histograms showing the expression of FCRL5 CD307e on non-transfected or Human FCRL5-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Middle and Right Plots: Tonsil cells were treated with an Fc receptor blocker and stained with APC Mouse Anti-Human CD19 Cat. No. 561742 or 555415 and either PE Mouse Anti-Human FCRL5 CD307e Cat. No. 566734; Middle Plot or PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of FCRL5 CD307e or Ig isotype control staining versus Human CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCalibur Flow Cytometer System and FlowJo software. Data courtesy of Dr. Marco Colonna, Washington University School of Medicine. Data shown on this Technical Data Sheet are not lot specific.