BD PharmingenTM PE Mouse anti-Human FoxA2

Description

Flow cytometric analysis of FoxA2 in definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days DAmour et al, 2005 in RPMI medium supplemented with 0.5 % FBS, 1x L-glutamine, and 100 ng/ml Activin A R&D Systems . Control ES cells left panel and day-3 differentiated cells right panel were fixed with BD Cytofix buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed lines, Cat. No.554680 or PE Mouse Anti-human FoxA2 antibody solid lines at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of the human ES and H9-derived endoderm cells, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody can also be used on fixed cells permeabilized with 0.1 % Triton X-100. Flow cytometric analysis of FoxA2 in definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days DAmour et al, 2005 in RPMI medium supplemented with 0.5 % FBS, 1x L-glutamine, and 100 ng/ml Activin A R&D Systems . Control ES cells left panel and day-3 differentiated cells right panel were fixed with BD Cytofix buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed lines, Cat. No.554680 or PE Mouse Anti-human FoxA2 antibody solid lines at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of the human ES and H9-derived endoderm cells, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody can also be used on fixed cells permeabilized with 0.1 % Triton X-100. Flow cytometric analysis of FoxA2 in definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days DAmour et al, 2005 in RPMI medium supplemented with 0.5 % FBS, 1x L-glutamine, and 100 ng/ml Activin A R&D Systems . Control ES cells left panel and day-3 differentiated cells right panel were fixed with BD Cytofix buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed lines, Cat. No.554680 or PE Mouse Anti-human FoxA2 antibody solid lines at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of the human ES and H9-derived endoderm cells, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody can also be used on fixed cells permeabilized with 0.1 % Triton X-100. Show More Flow cytometric analysis of FoxA2 in definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days DAmour et al, 2005 in RPMI medium supplemented with 0.5 % FBS, 1x L-glutamine, and 100 ng/ml Activin A R&D Systems . Control ES cells left panel and day-3 differentiated cells right panel were fixed with BD Cytofix buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed lines, Cat. No.554680 or PE Mouse Anti-human FoxA2 antibody solid lines at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of the human ES and H9-derived endoderm cells, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody can also be used on fixed cells permeabilized with 0.1 % Triton X-100. Show More Flow cytometric analysis of FoxA2 in definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days DAmour et al, 2005 in RPMI medium supplemented with 0.5 % FBS, 1x L-glutamine, and 100 ng/ml Activin A R&D Systems . Control ES cells left panel and day-3 differentiated cells right panel were fixed with BD Cytofix buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed lines, Cat. No.554680 or PE Mouse Anti-human FoxA2 antibody solid lines at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of the human ES and H9-derived endoderm cells, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody can also be used on fixed cells permeabilized with 0.1 % Triton X-100. Flow cytometric analysis of FoxA2 in definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days DAmour et al, 2005 in RPMI medium supplemented with 0.5 % FBS, 1x L-glutamine, and 100 ng/ml Activin A R&D Systems . Control ES cells left panel and day-3 differentiated cells right panel were fixed with BD Cytofix buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed lines, Cat. No.554680 or PE Mouse Anti-human FoxA2 antibody solid lines at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of the human ES and H9-derived endoderm cells, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody can also be used on fixed cells permeabilized with 0.1 % Triton X-100.