BD PharmingenTM PE Mouse anti-Human FoxP3

Description

Flow cytometric analysis of PE anti-human FoxP3 on resting PBMC. Human PBMC were stained with FITC anti-human CD4 clone RPA-T4, Cat. No. 555346 and APC anti-human CD25 clone M-A251, Cat. No. 555434 shown here or with clone 2A3, Cat. 340938, data not shown simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with PE anti-human FoxP3 clone 259D/C7; Cat No. 560046/560082 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of PE anti-human FoxP3 on resting PBMC. Human PBMC were stained with FITC anti-human CD4 clone RPA-T4, Cat. No. 555346 and APC anti-human CD25 clone M-A251, Cat. No. 555434 shown here or with clone 2A3, Cat. 340938, data not shown simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with PE anti-human FoxP3 clone 259D/C7; Cat No. 560046/560082 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of PE anti-human FoxP3 on resting PBMC. Human PBMC were stained with FITC anti-human CD4 clone RPA-T4, Cat. No. 555346 and APC anti-human CD25 clone M-A251, Cat. No. 555434 shown here or with clone 2A3, Cat. 340938, data not shown simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with PE anti-human FoxP3 clone 259D/C7; Cat No. 560046/560082 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of PE anti-human FoxP3 on resting PBMC. Human PBMC were stained with FITC anti-human CD4 clone RPA-T4, Cat. No. 555346 and APC anti-human CD25 clone M-A251, Cat. No. 555434 shown here or with clone 2A3, Cat. 340938, data not shown simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with PE anti-human FoxP3 clone 259D/C7; Cat No. 560046/560082 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of PE anti-human FoxP3 on resting PBMC. Human PBMC were stained with FITC anti-human CD4 clone RPA-T4, Cat. No. 555346 and APC anti-human CD25 clone M-A251, Cat. No. 555434 shown here or with clone 2A3, Cat. 340938, data not shown simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with PE anti-human FoxP3 clone 259D/C7; Cat No. 560046/560082 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of PE anti-human FoxP3 on resting PBMC. Human PBMC were stained with FITC anti-human CD4 clone RPA-T4, Cat. No. 555346 and APC anti-human CD25 clone M-A251, Cat. No. 555434 shown here or with clone 2A3, Cat. 340938, data not shown simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with PE anti-human FoxP3 clone 259D/C7; Cat No. 560046/560082 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System.