BD PharmingenTM PE Mouse anti-Human GARP

Description

Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were activated 12 hours with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 . The activated PBMC were washed and stained with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 556656 or PE Mouse Anti-Human GARP Cat. No. 562150 . The cells were then washed and stained with BD Horizon V450 Mouse Anti-Human FoxP3 Cat. No. 560459 using FoxP3 staining protocol. Two-color flow cytometric dot plots showing the correlated expression staining patterns of V450 FoxP3 versus PE anti-Human GARP right panel or PE Mouse IgG2b, kappa isotype control left panel were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were activated 12 hours with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 . The activated PBMC were washed and stained with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 556656 or PE Mouse Anti-Human GARP Cat. No. 562150 . The cells were then washed and stained with BD Horizon V450 Mouse Anti-Human FoxP3 Cat. No. 560459 using FoxP3 staining protocol. Two-color flow cytometric dot plots showing the correlated expression staining patterns of V450 FoxP3 versus PE anti-Human GARP right panel or PE Mouse IgG2b, kappa isotype control left panel were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were activated 12 hours with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 . The activated PBMC were washed and stained with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 556656 or PE Mouse Anti-Human GARP Cat. No. 562150 . The cells were then washed and stained with BD Horizon V450 Mouse Anti-Human FoxP3 Cat. No. 560459 using FoxP3 staining protocol. Two-color flow cytometric dot plots showing the correlated expression staining patterns of V450 FoxP3 versus PE anti-Human GARP right panel or PE Mouse IgG2b, kappa isotype control left panel were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were activated 12 hours with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 . The activated PBMC were washed and stained with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 556656 or PE Mouse Anti-Human GARP Cat. No. 562150 . The cells were then washed and stained with BD Horizon V450 Mouse Anti-Human FoxP3 Cat. No. 560459 using FoxP3 staining protocol. Two-color flow cytometric dot plots showing the correlated expression staining patterns of V450 FoxP3 versus PE anti-Human GARP right panel or PE Mouse IgG2b, kappa isotype control left panel were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were activated 12 hours with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 . The activated PBMC were washed and stained with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 556656 or PE Mouse Anti-Human GARP Cat. No. 562150 . The cells were then washed and stained with BD Horizon V450 Mouse Anti-Human FoxP3 Cat. No. 560459 using FoxP3 staining protocol. Two-color flow cytometric dot plots showing the correlated expression staining patterns of V450 FoxP3 versus PE anti-Human GARP right panel or PE Mouse IgG2b, kappa isotype control left panel were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were activated 12 hours with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 . The activated PBMC were washed and stained with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 556656 or PE Mouse Anti-Human GARP Cat. No. 562150 . The cells were then washed and stained with BD Horizon V450 Mouse Anti-Human FoxP3 Cat. No. 560459 using FoxP3 staining protocol. Two-color flow cytometric dot plots showing the correlated expression staining patterns of V450 FoxP3 versus PE anti-Human GARP right panel or PE Mouse IgG2b, kappa isotype control left panel were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.