BD PharmingenTM PE Mouse Anti-Human GITR (CD357)

Description

Multiparameter flow cytometric analysis of GITR CD357 expression on human peripheral blood lymphocytes. PBMC were cultured for 3 days in medium without Unstimulated; Left Plot or with Phytohemagglutinin-M PHA-M Stimulated; 1.5 % v/v; Right Plot . Cells were stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; no staining, data not shown or PE Mouse Anti-Human GITR CD357 antibody Cat. No. 567191/567192 . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of GITR CD357 versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD human peripheral blood lymphocytes. Multiparameter flow cytometric analysis of GITR CD357 expression on human CD4+ peripheral blood lymphocytes. PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 and APC Mouse Anti-Human CD25 Cat. No. 555434 antibodies and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; dashed line histograms or PE Mouse Anti-Human GITR CD357 antibody Cat. No. 567191/567192; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution was added to cells right before analysis. Histograms showing the expression of GITR CD357 or Ig Isotype control staining were derived from CD4+CD25-negative Left Plot or CD4+CD25-bright positive Right Plot gated events with the forward and side light-scatter characteristics of viable 7-AAD negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of GITR CD357 expression on human peripheral blood lymphocytes. PBMC were cultured for 3 days in medium without Unstimulated; Left Plot or with Phytohemagglutinin-M PHA-M Stimulated; 1.5 % v/v; Right Plot . Cells were stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; no staining, data not shown or PE Mouse Anti-Human GITR CD357 antibody Cat. No. 567191/567192 . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of GITR CD357 versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD human peripheral blood lymphocytes. Multiparameter flow cytometric analysis of GITR CD357 expression on human CD4+ peripheral blood lymphocytes. PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 and APC Mouse Anti-Human CD25 Cat. No. 555434 antibodies and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; dashed line histograms or PE Mouse Anti-Human GITR CD357 antibody Cat. No. 567191/567192; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution was added to cells right before analysis. Histograms showing the expression of GITR CD357 or Ig Isotype control staining were derived from CD4+CD25-negative Left Plot or CD4+CD25-bright positive Right Plot gated events with the forward and side light-scatter characteristics of viable 7-AAD negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of GITR CD357 expression on human peripheral blood lymphocytes. PBMC were cultured for 3 days in medium without Unstimulated; Left Plot or with Phytohemagglutinin-M PHA-M Stimulated; 1.5 % v/v; Right Plot . Cells were stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; no staining, data not shown or PE Mouse Anti-Human GITR CD357 antibody Cat. No. 567191/567192 . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of GITR CD357 versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD human peripheral blood lymphocytes. Multiparameter flow cytometric analysis of GITR CD357 expression on human CD4+ peripheral blood lymphocytes. PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 and APC Mouse Anti-Human CD25 Cat. No. 555434 antibodies and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; dashed line histograms or PE Mouse Anti-Human GITR CD357 antibody Cat. No. 567191/567192; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution was added to cells right before analysis. Histograms showing the expression of GITR CD357 or Ig Isotype control staining were derived from CD4+CD25-negative Left Plot or CD4+CD25-bright positive Right Plot gated events with the forward and side light-scatter characteristics of viable 7-AAD negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.