BD PharmingenTM PE Mouse Anti-Human Granzyme A

Description

Multiparameter flow cytometric analysis of Granzyme A expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Granzyme A antibody Cat. No. 568388/568389; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of Granzyme A expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Granzyme A antibody Cat. No. 568388/568389; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of Granzyme A expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Granzyme A antibody Cat. No. 568388/568389; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of Granzyme A expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Granzyme A antibody Cat. No. 568388/568389; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of Granzyme A expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Granzyme A antibody Cat. No. 568388/568389; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of Granzyme A expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Granzyme A antibody Cat. No. 568388/568389; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.