BD PharmingenTM PE Mouse Anti-Human HLA-A3

Description

Multiparameter flow cytometric analysis of HLA-A3 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Left Plot or PE Mouse Anti-Human HLA-A3 antibody Cat. No. 566605; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-parameter flow cytometric contour plots showing the correlated expression of HLA-A3 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Flow cytometric analysis of HLA-A3 expression on Jurkat cells. Jurkat cells were stained with PE Mouse IgG2a, kappa Isotype Control dashed line histogram or PE Mouse Anti-Human HLA-A3 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing HLA-A3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of HLA-A3 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Left Plot or PE Mouse Anti-Human HLA-A3 antibody Cat. No. 566605; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-parameter flow cytometric contour plots showing the correlated expression of HLA-A3 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Show More Flow cytometric analysis of HLA-A3 expression on Jurkat cells. Jurkat cells were stained with PE Mouse IgG2a, kappa Isotype Control dashed line histogram or PE Mouse Anti-Human HLA-A3 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing HLA-A3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of HLA-A3 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Left Plot or PE Mouse Anti-Human HLA-A3 antibody Cat. No. 566605; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-parameter flow cytometric contour plots showing the correlated expression of HLA-A3 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Flow cytometric analysis of HLA-A3 expression on Jurkat cells. Jurkat cells were stained with PE Mouse IgG2a, kappa Isotype Control dashed line histogram or PE Mouse Anti-Human HLA-A3 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing HLA-A3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.