BD PharmingenTM PE Mouse Anti-Human HLA-B7

Description

Multiparameter flow cytometric analysis of HLA-B7 expression on human peripheral blood leucocyte populations from a HLA-B7-positive donor. Human whole blood from a HLA-B7-positive donor was stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human HLA-B7 antibody Cat. No. 566654; Right Plot at 1 mug/ml. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-parameter pseudocolor dot plots showing the correlated expression of HLA-B7 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of HLA-B7 expression on Jurkat cells. Jurkat cells Acute T cell leukemia, ATCC TIB-152 were stained with either PE Mouse IgG1, kappa Isotype Control dashed line histogram or PE Mouse Anti-Human HLA-B7 antibody solid line histogram at 1 mug/ml. Florescence histograms showing HLA-B7 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of HLA-B7 expression on human peripheral blood leucocyte populations from a HLA-B7-positive donor. Human whole blood from a HLA-B7-positive donor was stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human HLA-B7 antibody Cat. No. 566654; Right Plot at 1 mug/ml. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-parameter pseudocolor dot plots showing the correlated expression of HLA-B7 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of HLA-B7 expression on Jurkat cells. Jurkat cells Acute T cell leukemia, ATCC TIB-152 were stained with either PE Mouse IgG1, kappa Isotype Control dashed line histogram or PE Mouse Anti-Human HLA-B7 antibody solid line histogram at 1 mug/ml. Florescence histograms showing HLA-B7 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of HLA-B7 expression on human peripheral blood leucocyte populations from a HLA-B7-positive donor. Human whole blood from a HLA-B7-positive donor was stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human HLA-B7 antibody Cat. No. 566654; Right Plot at 1 mug/ml. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-parameter pseudocolor dot plots showing the correlated expression of HLA-B7 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of HLA-B7 expression on Jurkat cells. Jurkat cells Acute T cell leukemia, ATCC TIB-152 were stained with either PE Mouse IgG1, kappa Isotype Control dashed line histogram or PE Mouse Anti-Human HLA-B7 antibody solid line histogram at 1 mug/ml. Florescence histograms showing HLA-B7 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.