BD PharmingenTM PE Mouse Anti-Human HLA-DR4

Description

Multiparameter flow cytometric analysis of HLA-DR4 expression on Human peripheral blood leukocyte populations. Human peripheral blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were stained with either BD Pharmingen PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or BD Pharmingen PE Mouse Anti-Human HLA-DR4 antibody Cat. No. 571188/571189; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of HLA-DR4 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of HLA-DR4 expression on Human peripheral blood lymphocytes. Human peripheral blood cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440 and with either BD Pharmingen PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or BD Pharmingen PE Mouse Anti-Human HLA-DR4 antibody Cat. No. 571188/571189; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of HLA-DR4 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of HLA-DR4 expression on Human peripheral blood leukocyte populations. Human peripheral blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were stained with either BD Pharmingen PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or BD Pharmingen PE Mouse Anti-Human HLA-DR4 antibody Cat. No. 571188/571189; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of HLA-DR4 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of HLA-DR4 expression on Human peripheral blood lymphocytes. Human peripheral blood cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440 and with either BD Pharmingen PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or BD Pharmingen PE Mouse Anti-Human HLA-DR4 antibody Cat. No. 571188/571189; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of HLA-DR4 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of HLA-DR4 expression on Human peripheral blood leukocyte populations. Human peripheral blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were stained with either BD Pharmingen PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or BD Pharmingen PE Mouse Anti-Human HLA-DR4 antibody Cat. No. 571188/571189; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of HLA-DR4 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of HLA-DR4 expression on Human peripheral blood lymphocytes. Human peripheral blood cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440 and with either BD Pharmingen PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or BD Pharmingen PE Mouse Anti-Human HLA-DR4 antibody Cat. No. 571188/571189; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of HLA-DR4 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.