BD PharmingenTM PE Mouse Anti-Human IDO1

Description

Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD11b Cat. No. 562632 and PE Mouse Anti-Human IDO1 antibody Cat. No. 567867/567868 . Bivariate pseudocolor density plots showing the correlated expression of IDO1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD11b Cat. No. 562632 and PE Mouse Anti-Human IDO1 antibody Cat. No. 567867/567868 . Bivariate pseudocolor density plots showing the correlated expression of IDO1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD11b Cat. No. 562632 and PE Mouse Anti-Human IDO1 antibody Cat. No. 567867/567868 . Bivariate pseudocolor density plots showing the correlated expression of IDO1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Show More Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD11b Cat. No. 562632 and PE Mouse Anti-Human IDO1 antibody Cat. No. 567867/567868 . Bivariate pseudocolor density plots showing the correlated expression of IDO1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Show More Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD11b Cat. No. 562632 and PE Mouse Anti-Human IDO1 antibody Cat. No. 567867/567868 . Bivariate pseudocolor density plots showing the correlated expression of IDO1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD11b Cat. No. 562632 and PE Mouse Anti-Human IDO1 antibody Cat. No. 567867/567868 . Bivariate pseudocolor density plots showing the correlated expression of IDO1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System.