BD PharmingenTM PE Mouse Anti-Human IFN-gamma

Description

Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 h with PMA 50 ng/ml; Sigma, Cat. #P-8139 and calcium ionophore A23187 250 ng/ml; Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. 555334 , fixed, permeabilized, and subsequently stained with 20 mul of PE Mouse Anti-Human IFN-gamma Cat. No. 559327/554701; left panel . To demonstrate specificity of staining, binding by the PE-B27 antibody was blocked by preincubation of fixed/permeabilized cells with Purified Mouse Anti-Human IFN-gamma 5 mug; Cat. No. 554699/550011; right panel prior to staining. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls and verified using the unlabeled antibody blocking control. Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 h with PMA 50 ng/ml; Sigma, Cat. #P-8139 and calcium ionophore A23187 250 ng/ml; Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. 555334 , fixed, permeabilized, and subsequently stained with 20 mul of PE Mouse Anti-Human IFN-gamma Cat. No. 559327/554701; left panel . To demonstrate specificity of staining, binding by the PE-B27 antibody was blocked by preincubation of fixed/permeabilized cells with Purified Mouse Anti-Human IFN-gamma 5 mug; Cat. No. 554699/550011; right panel prior to staining. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls and verified using the unlabeled antibody blocking control. Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 h with PMA 50 ng/ml; Sigma, Cat. #P-8139 and calcium ionophore A23187 250 ng/ml; Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. 555334 , fixed, permeabilized, and subsequently stained with 20 mul of PE Mouse Anti-Human IFN-gamma Cat. No. 559327/554701; left panel . To demonstrate specificity of staining, binding by the PE-B27 antibody was blocked by preincubation of fixed/permeabilized cells with Purified Mouse Anti-Human IFN-gamma 5 mug; Cat. No. 554699/550011; right panel prior to staining. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls and verified using the unlabeled antibody blocking control. Show More Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 h with PMA 50 ng/ml; Sigma, Cat. #P-8139 and calcium ionophore A23187 250 ng/ml; Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. 555334 , fixed, permeabilized, and subsequently stained with 20 mul of PE Mouse Anti-Human IFN-gamma Cat. No. 559327/554701; left panel . To demonstrate specificity of staining, binding by the PE-B27 antibody was blocked by preincubation of fixed/permeabilized cells with Purified Mouse Anti-Human IFN-gamma 5 mug; Cat. No. 554699/550011; right panel prior to staining. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls and verified using the unlabeled antibody blocking control. Show More Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 h with PMA 50 ng/ml; Sigma, Cat. #P-8139 and calcium ionophore A23187 250 ng/ml; Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. 555334 , fixed, permeabilized, and subsequently stained with 20 mul of PE Mouse Anti-Human IFN-gamma Cat. No. 559327/554701; left panel . To demonstrate specificity of staining, binding by the PE-B27 antibody was blocked by preincubation of fixed/permeabilized cells with Purified Mouse Anti-Human IFN-gamma 5 mug; Cat. No. 554699/550011; right panel prior to staining. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls and verified using the unlabeled antibody blocking control. Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 h with PMA 50 ng/ml; Sigma, Cat. #P-8139 and calcium ionophore A23187 250 ng/ml; Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. 555334 , fixed, permeabilized, and subsequently stained with 20 mul of PE Mouse Anti-Human IFN-gamma Cat. No. 559327/554701; left panel . To demonstrate specificity of staining, binding by the PE-B27 antibody was blocked by preincubation of fixed/permeabilized cells with Purified Mouse Anti-Human IFN-gamma 5 mug; Cat. No. 554699/550011; right panel prior to staining. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls and verified using the unlabeled antibody blocking control.