BD PharmingenTM PE Mouse Anti-Human IgA1

Description

Multicolor flow cytometric analysis of IgA1 expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or BD Pharmingen PE Mouse Anti-Human IgA1 antibody Cat. No. 571774/571775; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgA1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of IgA1 expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or BD Pharmingen PE Mouse Anti-Human IgA1 antibody Cat. No. 571774/571775; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgA1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of IgA1 expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or BD Pharmingen PE Mouse Anti-Human IgA1 antibody Cat. No. 571774/571775; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgA1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of IgA1 expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or BD Pharmingen PE Mouse Anti-Human IgA1 antibody Cat. No. 571774/571775; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgA1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of IgA1 expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or BD Pharmingen PE Mouse Anti-Human IgA1 antibody Cat. No. 571774/571775; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgA1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of IgA1 expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or BD Pharmingen PE Mouse Anti-Human IgA1 antibody Cat. No. 571774/571775; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgA1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.