BD PharmingenTM PE Mouse Anti-Human IgG1

Description

Two-color flow cytometric analysis of IgG1 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plot or PE Mouse Anti-Human IgG1 antibody Cat. No. 568275; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software. Two-color flow cytometric analysis of IgG1 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plot or PE Mouse Anti-Human IgG1 antibody Cat. No. 568275; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software. Two-color flow cytometric analysis of IgG1 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plot or PE Mouse Anti-Human IgG1 antibody Cat. No. 568275; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software. Show More Two-color flow cytometric analysis of IgG1 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plot or PE Mouse Anti-Human IgG1 antibody Cat. No. 568275; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software. Show More Two-color flow cytometric analysis of IgG1 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plot or PE Mouse Anti-Human IgG1 antibody Cat. No. 568275; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software. Two-color flow cytometric analysis of IgG1 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plot or PE Mouse Anti-Human IgG1 antibody Cat. No. 568275; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG1 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software.