BD PharmingenTM PE Mouse Anti-Human IgG2

Description

Multicolor flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG2 antibody Cat. No. 568959/568960; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG2 antibody Cat. No. 568959/568960; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG2 antibody Cat. No. 568959/568960; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG2 antibody Cat. No. 568959/568960; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG2 antibody Cat. No. 568959/568960; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG2 antibody Cat. No. 568959/568960; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.