BD PharmingenTM PE Mouse Anti-Human IgG3

Description

Two-color flow cytometric analysis of IgG3 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG3 antibody Cat. No. 568296; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of IgG3 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG3 antibody Cat. No. 568296; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of IgG3 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG3 antibody Cat. No. 568296; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of IgG3 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG3 antibody Cat. No. 568296; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of IgG3 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG3 antibody Cat. No. 568296; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of IgG3 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse anti-Human CD19 antibody Cat. No. 555415 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 349043; Left Plot or PE Mouse Anti-Human IgG3 antibody Cat. No. 568296; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.