BD PharmingenTM PE Mouse anti-Human IL-17A
560486 · 100 Tests
BDB Bioscience · Cat: 560486
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of PE anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with PE anti-human IL-17A and FITC anti-human IFN-gamma Cat. No. 554700; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of PE anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with PE anti-human IL-17A and FITC anti-human IFN-gamma Cat. No. 554700; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of PE anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with PE anti-human IL-17A and FITC anti-human IFN-gamma Cat. No. 554700; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of PE anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with PE anti-human IL-17A and FITC anti-human IFN-gamma Cat. No. 554700; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of PE anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with PE anti-human IL-17A and FITC anti-human IFN-gamma Cat. No. 554700; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of PE anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with PE anti-human IL-17A and FITC anti-human IFN-gamma Cat. No. 554700; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System.