BD PharmingenTM PE Mouse Anti-Human IL-1alpha

Description

Expression of IL-alpha by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 10 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were stained with FITC-mouse anti-human CD14 antibody Clone M5E2, Cat. No. 555397 fixed/permeabilized, and then stained with 0.25 mug of PE-mouse anti-human IL-1alpha antibody PE-364-3B3-14, Cat. No. 554561 , left panel . The data reflects gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE-364-3B3-14 was blocked by preincubation of the PE-364-3B3-14 with a molar excess of recombinant human IL-1alpha middle panel , and by the preincubation of the fixed/permeabilized cells with an excess of the unlabeled cold 364-3B3-14 mouse antibody 5 mug; Cat. No. 551223; right panel . The quadrant markers for the bivariate dot plots were set based on the fixed/permeabilized unstained cell controls, and verified with the recombinant cytokine and cold antibody specificity controls. Expression of IL-alpha by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 10 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were stained with FITC-mouse anti-human CD14 antibody Clone M5E2, Cat. No. 555397 fixed/permeabilized, and then stained with 0.25 mug of PE-mouse anti-human IL-1alpha antibody PE-364-3B3-14, Cat. No. 554561 , left panel . The data reflects gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE-364-3B3-14 was blocked by preincubation of the PE-364-3B3-14 with a molar excess of recombinant human IL-1alpha middle panel , and by the preincubation of the fixed/permeabilized cells with an excess of the unlabeled cold 364-3B3-14 mouse antibody 5 mug; Cat. No. 551223; right panel . The quadrant markers for the bivariate dot plots were set based on the fixed/permeabilized unstained cell controls, and verified with the recombinant cytokine and cold antibody specificity controls. Expression of IL-alpha by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 10 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were stained with FITC-mouse anti-human CD14 antibody Clone M5E2, Cat. No. 555397 fixed/permeabilized, and then stained with 0.25 mug of PE-mouse anti-human IL-1alpha antibody PE-364-3B3-14, Cat. No. 554561 , left panel . The data reflects gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE-364-3B3-14 was blocked by preincubation of the PE-364-3B3-14 with a molar excess of recombinant human IL-1alpha middle panel , and by the preincubation of the fixed/permeabilized cells with an excess of the unlabeled cold 364-3B3-14 mouse antibody 5 mug; Cat. No. 551223; right panel . The quadrant markers for the bivariate dot plots were set based on the fixed/permeabilized unstained cell controls, and verified with the recombinant cytokine and cold antibody specificity controls. Show More Expression of IL-alpha by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 10 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were stained with FITC-mouse anti-human CD14 antibody Clone M5E2, Cat. No. 555397 fixed/permeabilized, and then stained with 0.25 mug of PE-mouse anti-human IL-1alpha antibody PE-364-3B3-14, Cat. No. 554561 , left panel . The data reflects gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE-364-3B3-14 was blocked by preincubation of the PE-364-3B3-14 with a molar excess of recombinant human IL-1alpha middle panel , and by the preincubation of the fixed/permeabilized cells with an excess of the unlabeled cold 364-3B3-14 mouse antibody 5 mug; Cat. No. 551223; right panel . The quadrant markers for the bivariate dot plots were set based on the fixed/permeabilized unstained cell controls, and verified with the recombinant cytokine and cold antibody specificity controls. Show More Expression of IL-alpha by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 10 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were stained with FITC-mouse anti-human CD14 antibody Clone M5E2, Cat. No. 555397 fixed/permeabilized, and then stained with 0.25 mug of PE-mouse anti-human IL-1alpha antibody PE-364-3B3-14, Cat. No. 554561 , left panel . The data reflects gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE-364-3B3-14 was blocked by preincubation of the PE-364-3B3-14 with a molar excess of recombinant human IL-1alpha middle panel , and by the preincubation of the fixed/permeabilized cells with an excess of the unlabeled cold 364-3B3-14 mouse antibody 5 mug; Cat. No. 551223; right panel . The quadrant markers for the bivariate dot plots were set based on the fixed/permeabilized unstained cell controls, and verified with the recombinant cytokine and cold antibody specificity controls. Expression of IL-alpha by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 10 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were stained with FITC-mouse anti-human CD14 antibody Clone M5E2, Cat. No. 555397 fixed/permeabilized, and then stained with 0.25 mug of PE-mouse anti-human IL-1alpha antibody PE-364-3B3-14, Cat. No. 554561 , left panel . The data reflects gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE-364-3B3-14 was blocked by preincubation of the PE-364-3B3-14 with a molar excess of recombinant human IL-1alpha middle panel , and by the preincubation of the fixed/permeabilized cells with an excess of the unlabeled cold 364-3B3-14 mouse antibody 5 mug; Cat. No. 551223; right panel . The quadrant markers for the bivariate dot plots were set based on the fixed/permeabilized unstained cell controls, and verified with the recombinant cytokine and cold antibody specificity controls.