BD PharmingenTM PE Mouse Anti-Human IL-1beta

Description

Flow cytometric analysis of IL-1beta expression in activated human monocytes. Human peripheral blood mononuclear cells were cultured with Recombinant Human IFN-gamma protein Cat. No. 554616/554617; 10 ng/ml for 2 h at 37C and then stimulated with lipopolysaccharide 1 mug of LPS/ml and BD GolgiStop Protein Transport Inhibitor containing Monensin 2 muM; Cat. No. 554724 overnight at 37C. Cells were harvested, washed, and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either PE Mouse IgG1 kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human IL-1beta antibody Cat. No. 567779/567780; solid line histogram . The fluorescence histogram showing IL-1beta expression or Ig Isotype control staining was derived from gated events with the side and forward light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of IL-1beta expression in activated human monocytes. Human peripheral blood mononuclear cells were cultured with Recombinant Human IFN-gamma protein Cat. No. 554616/554617; 10 ng/ml for 2 h at 37C and then stimulated with lipopolysaccharide 1 mug of LPS/ml and BD GolgiStop Protein Transport Inhibitor containing Monensin 2 muM; Cat. No. 554724 overnight at 37C. Cells were harvested, washed, and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either PE Mouse IgG1 kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human IL-1beta antibody Cat. No. 567779/567780; solid line histogram . The fluorescence histogram showing IL-1beta expression or Ig Isotype control staining was derived from gated events with the side and forward light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of IL-1beta expression in activated human monocytes. Human peripheral blood mononuclear cells were cultured with Recombinant Human IFN-gamma protein Cat. No. 554616/554617; 10 ng/ml for 2 h at 37C and then stimulated with lipopolysaccharide 1 mug of LPS/ml and BD GolgiStop Protein Transport Inhibitor containing Monensin 2 muM; Cat. No. 554724 overnight at 37C. Cells were harvested, washed, and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either PE Mouse IgG1 kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human IL-1beta antibody Cat. No. 567779/567780; solid line histogram . The fluorescence histogram showing IL-1beta expression or Ig Isotype control staining was derived from gated events with the side and forward light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.