BD PharmingenTM PE Mouse Anti-Human IL-33 Receptor (ST2)

Description

Flow cytometric analysis of IL-33 Receptor ST2 expression on KU812 cells. Cells from the human KU812 Chronic Myelogenous Leukemia, ATCC CRL-2099 cell line were cultured in the presence Left Plot or absence of serum Right Plot for 6 days. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IL-33 Receptor ST2 antibody Cat. No. 569314/569315; solid line histograms . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing IL-33 Receptor ST2 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IL-33 Receptor ST2 expression on KU812 cells. Cells from the human KU812 Chronic Myelogenous Leukemia, ATCC CRL-2099 cell line were cultured in the presence Left Plot or absence of serum Right Plot for 6 days. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IL-33 Receptor ST2 antibody Cat. No. 569314/569315; solid line histograms . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing IL-33 Receptor ST2 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IL-33 Receptor ST2 expression on KU812 cells. Cells from the human KU812 Chronic Myelogenous Leukemia, ATCC CRL-2099 cell line were cultured in the presence Left Plot or absence of serum Right Plot for 6 days. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IL-33 Receptor ST2 antibody Cat. No. 569314/569315; solid line histograms . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing IL-33 Receptor ST2 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of IL-33 Receptor ST2 expression on KU812 cells. Cells from the human KU812 Chronic Myelogenous Leukemia, ATCC CRL-2099 cell line were cultured in the presence Left Plot or absence of serum Right Plot for 6 days. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IL-33 Receptor ST2 antibody Cat. No. 569314/569315; solid line histograms . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing IL-33 Receptor ST2 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of IL-33 Receptor ST2 expression on KU812 cells. Cells from the human KU812 Chronic Myelogenous Leukemia, ATCC CRL-2099 cell line were cultured in the presence Left Plot or absence of serum Right Plot for 6 days. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IL-33 Receptor ST2 antibody Cat. No. 569314/569315; solid line histograms . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing IL-33 Receptor ST2 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IL-33 Receptor ST2 expression on KU812 cells. Cells from the human KU812 Chronic Myelogenous Leukemia, ATCC CRL-2099 cell line were cultured in the presence Left Plot or absence of serum Right Plot for 6 days. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IL-33 Receptor ST2 antibody Cat. No. 569314/569315; solid line histograms . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing IL-33 Receptor ST2 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.