BD PharmingenTM PE Mouse Anti-Human IL-3Ralpha (CD123)

Description

Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Human whole blood was stained with APC Mouse Anti-Human CD45RO Cat No. 559865; Bottom Plots and with either PE Mouse IgG1, kappa Isotype Control Cat No. 554680, Left Plots or PE Mouse Anti-Human IL-3Ralpha CD123 Cat No. 566919/566920; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat No. 555899 . Upper Plots: Two-parameter pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable human leucocytes. Lower Plots: Two-parameter pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Human whole blood was stained with APC Mouse Anti-Human CD45RO Cat No. 559865; Bottom Plots and with either PE Mouse IgG1, kappa Isotype Control Cat No. 554680, Left Plots or PE Mouse Anti-Human IL-3Ralpha CD123 Cat No. 566919/566920; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat No. 555899 . Upper Plots: Two-parameter pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable human leucocytes. Lower Plots: Two-parameter pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Human whole blood was stained with APC Mouse Anti-Human CD45RO Cat No. 559865; Bottom Plots and with either PE Mouse IgG1, kappa Isotype Control Cat No. 554680, Left Plots or PE Mouse Anti-Human IL-3Ralpha CD123 Cat No. 566919/566920; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat No. 555899 . Upper Plots: Two-parameter pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable human leucocytes. Lower Plots: Two-parameter pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.