BD PharmingenTM PE Mouse Anti-Human Integrin alpha1 (CD49a)

Description

Flow cytometric analysis of Integrin alpha1 CD49a expression on Human Leucocytes. Human whole blood was stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 568716/568717; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of Integrin alpha1 CD49a or Ig Isotype control staining versus side light-scatter signals SSC was derived from gated events with the light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Integrin alpha1 CD49a expression on HeLa cells. Cells from the human HeLa Adenocarcinoma, ATCC CCL-2 cell line were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histogram or PE Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 568716/568717; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing Integrin alpha1 CD49a expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Integrin alpha1 CD49a expression on Human Leucocytes. Human whole blood was stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 568716/568717; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of Integrin alpha1 CD49a or Ig Isotype control staining versus side light-scatter signals SSC was derived from gated events with the light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of Integrin alpha1 CD49a expression on HeLa cells. Cells from the human HeLa Adenocarcinoma, ATCC CCL-2 cell line were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histogram or PE Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 568716/568717; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing Integrin alpha1 CD49a expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of Integrin alpha1 CD49a expression on Human Leucocytes. Human whole blood was stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 568716/568717; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of Integrin alpha1 CD49a or Ig Isotype control staining versus side light-scatter signals SSC was derived from gated events with the light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Integrin alpha1 CD49a expression on HeLa cells. Cells from the human HeLa Adenocarcinoma, ATCC CCL-2 cell line were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histogram or PE Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 568716/568717; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing Integrin alpha1 CD49a expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software.