BD PharmingenTM PE Mouse Anti-Human IRF1

Description

Flow cytometric analysis of IRF1 expressed in HeLa cells. Cells from the human HeLa Cervical adenocarcinoma, ATCC CCL-2 cell line were either cultured overnight in complete tissue culture medium without Left Panel or with Right Panel Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 . The cells were harvested, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IRF1 antibody Cat. No. 566322; solid line histograms at 0.5 mug/test. Data shown on this Technical Data Sheet are not lot specific. The fluorescence histograms showing IRF1 expression or Ig Isotype control staining , were derived from gated events with the forward and side light-scatter characteristics of intact HeLa cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Flow cytometric analysis of IRF1 expressed in HeLa cells. Cells from the human HeLa Cervical adenocarcinoma, ATCC CCL-2 cell line were either cultured overnight in complete tissue culture medium without Left Panel or with Right Panel Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 . The cells were harvested, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IRF1 antibody Cat. No. 566322; solid line histograms at 0.5 mug/test. Data shown on this Technical Data Sheet are not lot specific. The fluorescence histograms showing IRF1 expression or Ig Isotype control staining , were derived from gated events with the forward and side light-scatter characteristics of intact HeLa cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Flow cytometric analysis of IRF1 expressed in HeLa cells. Cells from the human HeLa Cervical adenocarcinoma, ATCC CCL-2 cell line were either cultured overnight in complete tissue culture medium without Left Panel or with Right Panel Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 . The cells were harvested, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IRF1 antibody Cat. No. 566322; solid line histograms at 0.5 mug/test. Data shown on this Technical Data Sheet are not lot specific. The fluorescence histograms showing IRF1 expression or Ig Isotype control staining , were derived from gated events with the forward and side light-scatter characteristics of intact HeLa cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Show More Flow cytometric analysis of IRF1 expressed in HeLa cells. Cells from the human HeLa Cervical adenocarcinoma, ATCC CCL-2 cell line were either cultured overnight in complete tissue culture medium without Left Panel or with Right Panel Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 . The cells were harvested, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IRF1 antibody Cat. No. 566322; solid line histograms at 0.5 mug/test. Data shown on this Technical Data Sheet are not lot specific. The fluorescence histograms showing IRF1 expression or Ig Isotype control staining , were derived from gated events with the forward and side light-scatter characteristics of intact HeLa cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Show More Flow cytometric analysis of IRF1 expressed in HeLa cells. Cells from the human HeLa Cervical adenocarcinoma, ATCC CCL-2 cell line were either cultured overnight in complete tissue culture medium without Left Panel or with Right Panel Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 . The cells were harvested, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IRF1 antibody Cat. No. 566322; solid line histograms at 0.5 mug/test. Data shown on this Technical Data Sheet are not lot specific. The fluorescence histograms showing IRF1 expression or Ig Isotype control staining , were derived from gated events with the forward and side light-scatter characteristics of intact HeLa cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Flow cytometric analysis of IRF1 expressed in HeLa cells. Cells from the human HeLa Cervical adenocarcinoma, ATCC CCL-2 cell line were either cultured overnight in complete tissue culture medium without Left Panel or with Right Panel Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 . The cells were harvested, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human IRF1 antibody Cat. No. 566322; solid line histograms at 0.5 mug/test. Data shown on this Technical Data Sheet are not lot specific. The fluorescence histograms showing IRF1 expression or Ig Isotype control staining , were derived from gated events with the forward and side light-scatter characteristics of intact HeLa cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System.