BD PharmingenTM PE Mouse Anti-Human LAP

Description

Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with PerCP Mouse Anti-Human CD4 Cat. No. 347324 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human LAP Cat. No. 562260; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 488 Mouse Anti-Human FoxP3 Cat. No. 560047 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Intracellular staining for LAP on P3UI-TGFbeta1 transfectant has also been demonstrated. Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with PerCP Mouse Anti-Human CD4 Cat. No. 347324 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human LAP Cat. No. 562260; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 488 Mouse Anti-Human FoxP3 Cat. No. 560047 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Intracellular staining for LAP on P3UI-TGFbeta1 transfectant has also been demonstrated. Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with PerCP Mouse Anti-Human CD4 Cat. No. 347324 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human LAP Cat. No. 562260; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 488 Mouse Anti-Human FoxP3 Cat. No. 560047 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Intracellular staining for LAP on P3UI-TGFbeta1 transfectant has also been demonstrated. Show More Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with PerCP Mouse Anti-Human CD4 Cat. No. 347324 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human LAP Cat. No. 562260; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 488 Mouse Anti-Human FoxP3 Cat. No. 560047 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Intracellular staining for LAP on P3UI-TGFbeta1 transfectant has also been demonstrated. Show More Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with PerCP Mouse Anti-Human CD4 Cat. No. 347324 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human LAP Cat. No. 562260; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 488 Mouse Anti-Human FoxP3 Cat. No. 560047 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Intracellular staining for LAP on P3UI-TGFbeta1 transfectant has also been demonstrated. Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with PerCP Mouse Anti-Human CD4 Cat. No. 347324 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human LAP Cat. No. 562260; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 488 Mouse Anti-Human FoxP3 Cat. No. 560047 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Intracellular staining for LAP on P3UI-TGFbeta1 transfectant has also been demonstrated.