BD PharmingenTM PE Mouse Anti-Human MerTK (Mer)

Description

Flow cytometric analysis of MerTK Mer expression on viable Human U-937 cells. Cells from the U-937 Histiocytic lymphoma, ATCC CRL-1593 cell line were preincubated with Human BD Fc Block Cat. No. 564220 and then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histogram or PE Mouse Anti-Human MerTK Mer antibody Cat. No. 571778/571779, solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing MerTK Mer expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of MerTK Mer expression on viable Human monocyte-derived macrophages. Normal Human monocytes were cultured in complete RPMI-1640 medium with heat-inactivated fetal bovine serum and purified recombinant Human M-CSF protein 50 ng/ml; PeproTech for seven days. Dexamethasone 10 nM; Sigma-Aldrich was added for the final 3 days to promote M2 macrophage polarization. The macrophages were harvested and stained with APC Mouse Anti-Human CD11b/Mac-1 antibody Cat. No. 550019 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human MerTK Mer antibody Cat. No. 571778/571779, Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of MerTK Mer or Ig Isotype control staining versus were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of MerTK Mer expression on viable Human U-937 cells. Cells from the U-937 Histiocytic lymphoma, ATCC CRL-1593 cell line were preincubated with Human BD Fc Block Cat. No. 564220 and then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histogram or PE Mouse Anti-Human MerTK Mer antibody Cat. No. 571778/571779, solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing MerTK Mer expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of MerTK Mer expression on viable Human monocyte-derived macrophages. Normal Human monocytes were cultured in complete RPMI-1640 medium with heat-inactivated fetal bovine serum and purified recombinant Human M-CSF protein 50 ng/ml; PeproTech for seven days. Dexamethasone 10 nM; Sigma-Aldrich was added for the final 3 days to promote M2 macrophage polarization. The macrophages were harvested and stained with APC Mouse Anti-Human CD11b/Mac-1 antibody Cat. No. 550019 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human MerTK Mer antibody Cat. No. 571778/571779, Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of MerTK Mer or Ig Isotype control staining versus were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of MerTK Mer expression on viable Human U-937 cells. Cells from the U-937 Histiocytic lymphoma, ATCC CRL-1593 cell line were preincubated with Human BD Fc Block Cat. No. 564220 and then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histogram or PE Mouse Anti-Human MerTK Mer antibody Cat. No. 571778/571779, solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing MerTK Mer expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of MerTK Mer expression on viable Human monocyte-derived macrophages. Normal Human monocytes were cultured in complete RPMI-1640 medium with heat-inactivated fetal bovine serum and purified recombinant Human M-CSF protein 50 ng/ml; PeproTech for seven days. Dexamethasone 10 nM; Sigma-Aldrich was added for the final 3 days to promote M2 macrophage polarization. The macrophages were harvested and stained with APC Mouse Anti-Human CD11b/Mac-1 antibody Cat. No. 550019 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human MerTK Mer antibody Cat. No. 571778/571779, Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of MerTK Mer or Ig Isotype control staining versus were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software.