BD PharmingenTM PE Mouse anti-human Nanog

Description

Spontaneous differentiation of human ES H9 WiCell, Madison, WI . H9 cells, passage 31, grown in mTESR1 media StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were treated with human ES basal culture media that contained Knockout Serum Replacement LifeTechnologies and 10muM Retinoic Acid Sigma for 3 days. Cells were harvested with Accutase Innovative Cell Technologies on Day 0 left panel , Day 1 middle panel and Day 3 right panel , fixed In BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with PE anti-human Nanog solid line or PE Mouse IgG1, kappa Isotype Control Cat. No.554680 . Flow cytometry was performed on a BD LSR II flow cytometry system. This reagent will also work in BD Phosphlow Perm II and III buffers. Spontaneous differentiation of human ES H9 WiCell, Madison, WI . H9 cells, passage 31, grown in mTESR1 media StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were treated with human ES basal culture media that contained Knockout Serum Replacement LifeTechnologies and 10muM Retinoic Acid Sigma for 3 days. Cells were harvested with Accutase Innovative Cell Technologies on Day 0 left panel , Day 1 middle panel and Day 3 right panel , fixed In BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with PE anti-human Nanog solid line or PE Mouse IgG1, kappa Isotype Control Cat. No.554680 . Flow cytometry was performed on a BD LSR II flow cytometry system. This reagent will also work in BD Phosphlow Perm II and III buffers. Spontaneous differentiation of human ES H9 WiCell, Madison, WI . H9 cells, passage 31, grown in mTESR1 media StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were treated with human ES basal culture media that contained Knockout Serum Replacement LifeTechnologies and 10muM Retinoic Acid Sigma for 3 days. Cells were harvested with Accutase Innovative Cell Technologies on Day 0 left panel , Day 1 middle panel and Day 3 right panel , fixed In BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with PE anti-human Nanog solid line or PE Mouse IgG1, kappa Isotype Control Cat. No.554680 . Flow cytometry was performed on a BD LSR II flow cytometry system. This reagent will also work in BD Phosphlow Perm II and III buffers. Show More Spontaneous differentiation of human ES H9 WiCell, Madison, WI . H9 cells, passage 31, grown in mTESR1 media StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were treated with human ES basal culture media that contained Knockout Serum Replacement LifeTechnologies and 10muM Retinoic Acid Sigma for 3 days. Cells were harvested with Accutase Innovative Cell Technologies on Day 0 left panel , Day 1 middle panel and Day 3 right panel , fixed In BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with PE anti-human Nanog solid line or PE Mouse IgG1, kappa Isotype Control Cat. No.554680 . Flow cytometry was performed on a BD LSR II flow cytometry system. This reagent will also work in BD Phosphlow Perm II and III buffers. Show More Spontaneous differentiation of human ES H9 WiCell, Madison, WI . H9 cells, passage 31, grown in mTESR1 media StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were treated with human ES basal culture media that contained Knockout Serum Replacement LifeTechnologies and 10muM Retinoic Acid Sigma for 3 days. Cells were harvested with Accutase Innovative Cell Technologies on Day 0 left panel , Day 1 middle panel and Day 3 right panel , fixed In BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with PE anti-human Nanog solid line or PE Mouse IgG1, kappa Isotype Control Cat. No.554680 . Flow cytometry was performed on a BD LSR II flow cytometry system. This reagent will also work in BD Phosphlow Perm II and III buffers. Spontaneous differentiation of human ES H9 WiCell, Madison, WI . H9 cells, passage 31, grown in mTESR1 media StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were treated with human ES basal culture media that contained Knockout Serum Replacement LifeTechnologies and 10muM Retinoic Acid Sigma for 3 days. Cells were harvested with Accutase Innovative Cell Technologies on Day 0 left panel , Day 1 middle panel and Day 3 right panel , fixed In BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with PE anti-human Nanog solid line or PE Mouse IgG1, kappa Isotype Control Cat. No.554680 . Flow cytometry was performed on a BD LSR II flow cytometry system. This reagent will also work in BD Phosphlow Perm II and III buffers.