BD PharmingenTM PE Mouse Anti-Human Pre-BCR

Description

Flow cytometric analysis of total Pre-BCR expression by 697 cells . Cells from the Human 697 Pre-B leukemia cell line cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or with PE Mouse Anti-Human Pre-BCR antibody Cat. No. 569719/569720; solid line histogram at 0.125 mug/test. The fluorescence histogram showing cell surface and intracellular Pre-BCR expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Pre-BCR expression on viable Human 697 cells. Cells from the Human 697 Pre-B leukemia cell line were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human Pre-BCR antibody Cat. No. 569719/569720; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of Pre-BCR or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of total Pre-BCR expression by 697 cells . Cells from the Human 697 Pre-B leukemia cell line cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or with PE Mouse Anti-Human Pre-BCR antibody Cat. No. 569719/569720; solid line histogram at 0.125 mug/test. The fluorescence histogram showing cell surface and intracellular Pre-BCR expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Pre-BCR expression on viable Human 697 cells. Cells from the Human 697 Pre-B leukemia cell line were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human Pre-BCR antibody Cat. No. 569719/569720; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of Pre-BCR or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of total Pre-BCR expression by 697 cells . Cells from the Human 697 Pre-B leukemia cell line cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or with PE Mouse Anti-Human Pre-BCR antibody Cat. No. 569719/569720; solid line histogram at 0.125 mug/test. The fluorescence histogram showing cell surface and intracellular Pre-BCR expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Pre-BCR expression on viable Human 697 cells. Cells from the Human 697 Pre-B leukemia cell line were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human Pre-BCR antibody Cat. No. 569719/569720; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of Pre-BCR or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.