BD PharmingenTM PE Mouse anti-Human Sox1

Description

Analysis of Sox1 in H9 Embryonic Stem ES cells left panel , H9-derived Neural Stem Cells NSC middle panel , and H9-derived Neurons right panel . H9 human ES cells WiCell, Madison, WI , H9-derived NSC, and H9-derived neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . All cells were then blocked with 10 % mouse serum Sigma M9505 and stained with matching concentrations of either PE Mouse IgG1, kappa isotype control dashed histograms, Cat. No. 554680 or PE Mouse Anti-Human Sox1 monoclonal antibody solid lines . Histograms were derived from gated events based on light scattering characteristics of the ES, NSC, and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. In the right panel, the brighter peak consists of undifferentiated NSC and glial cells that are staining positive for Sox1 and the dimmer peak consists of the neuronal population. Analysis of Sox1 in H9 Embryonic Stem ES cells left panel , H9-derived Neural Stem Cells NSC middle panel , and H9-derived Neurons right panel . H9 human ES cells WiCell, Madison, WI , H9-derived NSC, and H9-derived neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . All cells were then blocked with 10 % mouse serum Sigma M9505 and stained with matching concentrations of either PE Mouse IgG1, kappa isotype control dashed histograms, Cat. No. 554680 or PE Mouse Anti-Human Sox1 monoclonal antibody solid lines . Histograms were derived from gated events based on light scattering characteristics of the ES, NSC, and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. In the right panel, the brighter peak consists of undifferentiated NSC and glial cells that are staining positive for Sox1 and the dimmer peak consists of the neuronal population. Analysis of Sox1 in H9 Embryonic Stem ES cells left panel , H9-derived Neural Stem Cells NSC middle panel , and H9-derived Neurons right panel . H9 human ES cells WiCell, Madison, WI , H9-derived NSC, and H9-derived neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . All cells were then blocked with 10 % mouse serum Sigma M9505 and stained with matching concentrations of either PE Mouse IgG1, kappa isotype control dashed histograms, Cat. No. 554680 or PE Mouse Anti-Human Sox1 monoclonal antibody solid lines . Histograms were derived from gated events based on light scattering characteristics of the ES, NSC, and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. In the right panel, the brighter peak consists of undifferentiated NSC and glial cells that are staining positive for Sox1 and the dimmer peak consists of the neuronal population. Show More Analysis of Sox1 in H9 Embryonic Stem ES cells left panel , H9-derived Neural Stem Cells NSC middle panel , and H9-derived Neurons right panel . H9 human ES cells WiCell, Madison, WI , H9-derived NSC, and H9-derived neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . All cells were then blocked with 10 % mouse serum Sigma M9505 and stained with matching concentrations of either PE Mouse IgG1, kappa isotype control dashed histograms, Cat. No. 554680 or PE Mouse Anti-Human Sox1 monoclonal antibody solid lines . Histograms were derived from gated events based on light scattering characteristics of the ES, NSC, and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. In the right panel, the brighter peak consists of undifferentiated NSC and glial cells that are staining positive for Sox1 and the dimmer peak consists of the neuronal population. Show More Analysis of Sox1 in H9 Embryonic Stem ES cells left panel , H9-derived Neural Stem Cells NSC middle panel , and H9-derived Neurons right panel . H9 human ES cells WiCell, Madison, WI , H9-derived NSC, and H9-derived neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . All cells were then blocked with 10 % mouse serum Sigma M9505 and stained with matching concentrations of either PE Mouse IgG1, kappa isotype control dashed histograms, Cat. No. 554680 or PE Mouse Anti-Human Sox1 monoclonal antibody solid lines . Histograms were derived from gated events based on light scattering characteristics of the ES, NSC, and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. In the right panel, the brighter peak consists of undifferentiated NSC and glial cells that are staining positive for Sox1 and the dimmer peak consists of the neuronal population. Analysis of Sox1 in H9 Embryonic Stem ES cells left panel , H9-derived Neural Stem Cells NSC middle panel , and H9-derived Neurons right panel . H9 human ES cells WiCell, Madison, WI , H9-derived NSC, and H9-derived neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . All cells were then blocked with 10 % mouse serum Sigma M9505 and stained with matching concentrations of either PE Mouse IgG1, kappa isotype control dashed histograms, Cat. No. 554680 or PE Mouse Anti-Human Sox1 monoclonal antibody solid lines . Histograms were derived from gated events based on light scattering characteristics of the ES, NSC, and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. In the right panel, the brighter peak consists of undifferentiated NSC and glial cells that are staining positive for Sox1 and the dimmer peak consists of the neuronal population.