
564965 ·
BDB Bioscience · Cat: 564965
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 followed by permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with BD Horizon BV421 Mouse Anti-Human CD20 antibody Cat. No. 563346 and either PE IgG1, kappa Isotype Control Cat. No. 554680, Top Panels or PE Mouse Anti-Human STING antibody Cat. No. 564965; Bottom Panels . Left Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus Side Light Scatter SSC were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 followed by permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with BD Horizon BV421 Mouse Anti-Human CD20 antibody Cat. No. 563346 and either PE IgG1, kappa Isotype Control Cat. No. 554680, Top Panels or PE Mouse Anti-Human STING antibody Cat. No. 564965; Bottom Panels . Left Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus Side Light Scatter SSC were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 followed by permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with BD Horizon BV421 Mouse Anti-Human CD20 antibody Cat. No. 563346 and either PE IgG1, kappa Isotype Control Cat. No. 554680, Top Panels or PE Mouse Anti-Human STING antibody Cat. No. 564965; Bottom Panels . Left Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus Side Light Scatter SSC were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.