BD PharmingenTM PE Mouse Anti-Human TCR Vbeta5.1

Description

Two-color flow cytometric analysis of TCR Vbeta5.1 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human TCR Vbeta5.1 antibody Cat. No. 567042/567043; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCR Vbeta5.1 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human TCR Vbeta5.1 antibody Cat. No. 567042/567043; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCR Vbeta5.1 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human TCR Vbeta5.1 antibody Cat. No. 567042/567043; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of TCR Vbeta5.1 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human TCR Vbeta5.1 antibody Cat. No. 567042/567043; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of TCR Vbeta5.1 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human TCR Vbeta5.1 antibody Cat. No. 567042/567043; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCR Vbeta5.1 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human TCR Vbeta5.1 antibody Cat. No. 567042/567043; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.