BD PharmingenTM PE Mouse Anti-Human TIGIT

Description

Multicolor flow cytometric analysis of TIGIT expression on peripheral blood leucocytes. Whole blood was stained with Alexa Fluor 647 Mouse Anti-Human CD56 Cat. No. 563443; Middle Plots , BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , and BD Horizon BUV395 Mouse Anti-Human CD45RO Cat. No. 564291/564292; Right Plots antibodies, and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Top Plots or PE Mouse Anti-Human TIGIT antibody Cat. No. 568672/568673; Bottom Plots as indicated. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 was derived from gated events with the light scatter characteristics of viable lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the light scatter characteristics of viable lymphocytes. Multicolor flow cytometric analysis of TIGIT expression on peripheral blood leucocytes. Whole blood was stained with Alexa Fluor 647 Mouse Anti-Human CD56 Cat. No. 563443; Middle Plots , BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , and BD Horizon BUV395 Mouse Anti-Human CD45RO Cat. No. 564291/564292; Right Plots antibodies, and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Top Plots or PE Mouse Anti-Human TIGIT antibody Cat. No. 568672/568673; Bottom Plots as indicated. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 was derived from gated events with the light scatter characteristics of viable lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the light scatter characteristics of viable lymphocytes. Show More Multicolor flow cytometric analysis of TIGIT expression on peripheral blood leucocytes. Whole blood was stained with Alexa Fluor 647 Mouse Anti-Human CD56 Cat. No. 563443; Middle Plots , BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , and BD Horizon BUV395 Mouse Anti-Human CD45RO Cat. No. 564291/564292; Right Plots antibodies, and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Top Plots or PE Mouse Anti-Human TIGIT antibody Cat. No. 568672/568673; Bottom Plots as indicated. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 was derived from gated events with the light scatter characteristics of viable lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the light scatter characteristics of viable lymphocytes.