BD PharmingenTM PE Mouse Anti-Human TNF
557068 ·
BDB Bioscience · Cat: 557068
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Expression of TNF protein by stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus macaque PBMC were stimulated for 6 hours with PMA 50 ng/ml final concentration; Sigma, Cat. #P-8139 , calcium ionophore A23187 0.5 mug/ml final concentration; Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with 0.25 mug FITC Mouse Anti-Human CD3 Cat. No. 555332 and subsequently stained with 0.25 ug of PE Mouse Anti-Human TNF Cat. No. 557068/559321/554513/562083 . The quadrant markers for the bivariate dot plot was set based on the negative staining controls using isotype-matched Ig controls. Expression of TNF protein by stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus macaque PBMC were stimulated for 6 hours with PMA 50 ng/ml final concentration; Sigma, Cat. #P-8139 , calcium ionophore A23187 0.5 mug/ml final concentration; Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with 0.25 mug FITC Mouse Anti-Human CD3 Cat. No. 555332 and subsequently stained with 0.25 ug of PE Mouse Anti-Human TNF Cat. No. 557068/559321/554513/562083 . The quadrant markers for the bivariate dot plot was set based on the negative staining controls using isotype-matched Ig controls. Show More Expression of TNF protein by stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus macaque PBMC were stimulated for 6 hours with PMA 50 ng/ml final concentration; Sigma, Cat. #P-8139 , calcium ionophore A23187 0.5 mug/ml final concentration; Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with 0.25 mug FITC Mouse Anti-Human CD3 Cat. No. 555332 and subsequently stained with 0.25 ug of PE Mouse Anti-Human TNF Cat. No. 557068/559321/554513/562083 . The quadrant markers for the bivariate dot plot was set based on the negative staining controls using isotype-matched Ig controls.