BD PharmingenTM PE Mouse Anti-Human TNFR Related Protein (LTbetaR)

Description

Flow cytometric analysis of LTbetaR expression by HeLa, Jurkat and human monocytes. HeLa cell line Left Panel , Jurkat cell line Middle Panel , or human peripheral monocytes Right Panel were stained with PE Mouse Anti-Human TNFR Related Protein LTbetaR Cat No. 551503; filled histograms or PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; empty histograms at 0 .5 mug/106 cells. Whole human blood was first treated with PharmLyse Lysing Buffer Cat No. 555899 to lyse erythrocytes and blocked with normal polyclonal human IgG 5 mug/106 cells prior to staining. Note that certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometric analysis of LTbetaR expression by HeLa, Jurkat and human monocytes. HeLa cell line Left Panel , Jurkat cell line Middle Panel , or human peripheral monocytes Right Panel were stained with PE Mouse Anti-Human TNFR Related Protein LTbetaR Cat No. 551503; filled histograms or PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; empty histograms at 0 .5 mug/106 cells. Whole human blood was first treated with PharmLyse Lysing Buffer Cat No. 555899 to lyse erythrocytes and blocked with normal polyclonal human IgG 5 mug/106 cells prior to staining. Note that certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometric analysis of LTbetaR expression by HeLa, Jurkat and human monocytes. HeLa cell line Left Panel , Jurkat cell line Middle Panel , or human peripheral monocytes Right Panel were stained with PE Mouse Anti-Human TNFR Related Protein LTbetaR Cat No. 551503; filled histograms or PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; empty histograms at 0 .5 mug/106 cells. Whole human blood was first treated with PharmLyse Lysing Buffer Cat No. 555899 to lyse erythrocytes and blocked with normal polyclonal human IgG 5 mug/106 cells prior to staining. Note that certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Show More Flow cytometric analysis of LTbetaR expression by HeLa, Jurkat and human monocytes. HeLa cell line Left Panel , Jurkat cell line Middle Panel , or human peripheral monocytes Right Panel were stained with PE Mouse Anti-Human TNFR Related Protein LTbetaR Cat No. 551503; filled histograms or PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; empty histograms at 0 .5 mug/106 cells. Whole human blood was first treated with PharmLyse Lysing Buffer Cat No. 555899 to lyse erythrocytes and blocked with normal polyclonal human IgG 5 mug/106 cells prior to staining. Note that certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Show More Flow cytometric analysis of LTbetaR expression by HeLa, Jurkat and human monocytes. HeLa cell line Left Panel , Jurkat cell line Middle Panel , or human peripheral monocytes Right Panel were stained with PE Mouse Anti-Human TNFR Related Protein LTbetaR Cat No. 551503; filled histograms or PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; empty histograms at 0 .5 mug/106 cells. Whole human blood was first treated with PharmLyse Lysing Buffer Cat No. 555899 to lyse erythrocytes and blocked with normal polyclonal human IgG 5 mug/106 cells prior to staining. Note that certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometric analysis of LTbetaR expression by HeLa, Jurkat and human monocytes. HeLa cell line Left Panel , Jurkat cell line Middle Panel , or human peripheral monocytes Right Panel were stained with PE Mouse Anti-Human TNFR Related Protein LTbetaR Cat No. 551503; filled histograms or PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; empty histograms at 0 .5 mug/106 cells. Whole human blood was first treated with PharmLyse Lysing Buffer Cat No. 555899 to lyse erythrocytes and blocked with normal polyclonal human IgG 5 mug/106 cells prior to staining. Note that certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells.