BD PharmingenTM PE Mouse Anti-Human VEGFR2 (CD309)

Description

Flow cytometric analysis of Human VEGFR2 CD309 expression on viable HUVEC cells . Human Umbilical Vein Endothelial Cells HUVEC; Lonza, Cat. No. C2519A were grown in EGM-2 Endothelial Cell Growth Medium Lonza, Cat No. CC-3162 , which contains Vascular endothelial growth factor VEGF , and were dissociated with Reagent Pack Subculture Reagents Lonza, Cat No. CC-5034 . The cells were preincubated with Human BD Fc Block Cat. No. 564219 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human VEGFR2 CD309 antibody Cat. No. 571194/571200; solid line histograms . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing VEGFR2 CD309 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of Human VEGFR2 CD309 expression on viable HUVEC cells . Human Umbilical Vein Endothelial Cells HUVEC; Lonza, Cat. No. C2519A were grown in EGM-2 Endothelial Cell Growth Medium Lonza, Cat No. CC-3162 , which contains Vascular endothelial growth factor VEGF , and were dissociated with Reagent Pack Subculture Reagents Lonza, Cat No. CC-5034 . The cells were preincubated with Human BD Fc Block Cat. No. 564219 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human VEGFR2 CD309 antibody Cat. No. 571194/571200; solid line histograms . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing VEGFR2 CD309 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of Human VEGFR2 CD309 expression on viable HUVEC cells . Human Umbilical Vein Endothelial Cells HUVEC; Lonza, Cat. No. C2519A were grown in EGM-2 Endothelial Cell Growth Medium Lonza, Cat No. CC-3162 , which contains Vascular endothelial growth factor VEGF , and were dissociated with Reagent Pack Subculture Reagents Lonza, Cat No. CC-5034 . The cells were preincubated with Human BD Fc Block Cat. No. 564219 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Human VEGFR2 CD309 antibody Cat. No. 571194/571200; solid line histograms . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing VEGFR2 CD309 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.