BD PharmingenTM PE Mouse Anti-Human VISTA

Description

Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human VISTA antibody Cat. No. 566669/566672; Right Plot . Two-parameter flow cytometric contour plots showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human VISTA antibody Cat. No. 566669/566672; Right Plot . Two-parameter flow cytometric contour plots showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human VISTA antibody Cat. No. 566669/566672; Right Plot . Two-parameter flow cytometric contour plots showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human VISTA antibody Cat. No. 566669/566672; Right Plot . Two-parameter flow cytometric contour plots showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human VISTA antibody Cat. No. 566669/566672; Right Plot . Two-parameter flow cytometric contour plots showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human VISTA antibody Cat. No. 566669/566672; Right Plot . Two-parameter flow cytometric contour plots showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.