BD PharmingenTM PE Mouse Anti-Human ZBTB7B (ThPOK)

Description

Two-color flow cytometric analysis of ZBTB7B Th-POK expression in human peripheral blood mononuclear cells. PBMCs were surface stained with Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695 . The cells were then washed, fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human ZBTB7B ThPOK antibody. The two-color flow cytometric contour plots showing the correlated expression of ZBTB7B ThPOK or Ig Isotype Control versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR Fortessa X-20 Flow Cytometer System. Two-color flow cytometric analysis of ZBTB7B Th-POK expression in human peripheral blood mononuclear cells. PBMCs were surface stained with Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695 . The cells were then washed, fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human ZBTB7B ThPOK antibody. The two-color flow cytometric contour plots showing the correlated expression of ZBTB7B ThPOK or Ig Isotype Control versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR Fortessa X-20 Flow Cytometer System. Two-color flow cytometric analysis of ZBTB7B Th-POK expression in human peripheral blood mononuclear cells. PBMCs were surface stained with Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695 . The cells were then washed, fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human ZBTB7B ThPOK antibody. The two-color flow cytometric contour plots showing the correlated expression of ZBTB7B ThPOK or Ig Isotype Control versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR Fortessa X-20 Flow Cytometer System. Show More Two-color flow cytometric analysis of ZBTB7B Th-POK expression in human peripheral blood mononuclear cells. PBMCs were surface stained with Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695 . The cells were then washed, fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human ZBTB7B ThPOK antibody. The two-color flow cytometric contour plots showing the correlated expression of ZBTB7B ThPOK or Ig Isotype Control versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR Fortessa X-20 Flow Cytometer System. Show More Two-color flow cytometric analysis of ZBTB7B Th-POK expression in human peripheral blood mononuclear cells. PBMCs were surface stained with Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695 . The cells were then washed, fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human ZBTB7B ThPOK antibody. The two-color flow cytometric contour plots showing the correlated expression of ZBTB7B ThPOK or Ig Isotype Control versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR Fortessa X-20 Flow Cytometer System. Two-color flow cytometric analysis of ZBTB7B Th-POK expression in human peripheral blood mononuclear cells. PBMCs were surface stained with Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695 . The cells were then washed, fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680 or PE Mouse Anti-Human ZBTB7B ThPOK antibody. The two-color flow cytometric contour plots showing the correlated expression of ZBTB7B ThPOK or Ig Isotype Control versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR Fortessa X-20 Flow Cytometer System.